All lanes : Anti-DAP Kinase 2 antibody [1B10] (ab119035) at 1/2000 dilutionLane 1 : HEK293T cell lysate transfected with pCMV6-ENTRY controlLane 2 : HEK293T cell lysate transfected with pCMV6-ENTRY DAP Kinase 2 cDNALysates/proteins at 5 µg per lane.
ab119035, at 1/100 dilution, staining DAP Kianse 2 in COS7 cells transiently transfected with pCMV6-ENTRY DAP Kinase 2 by Immunofluorescence.
HEK293T cells transfected with either pCMV6-ENTRY DAP Kinase 2 (Red) or empty vector control plasmid (Blue) were immunostained using ab119035 at 1/100 dilution and then analyzed by flow cytometry.