DAPK2

Gene Symbol DAPK2
Entrez Gene 23604
Alt Symbol DRP-1, DRP1
Species Human
Gene Type protein-coding
Description death-associated protein kinase 2
Other Description DAP kinase 2|DAP-kinase-related protein 1 beta isoform
Swissprots Q24JS1 O75892 E9JGM7 Q9UIK4
Accessions EAW77657 EAW77658 EAW77659 Q9UIK4 AB018001 BAA88063 AF052941 AAC35001 AK026801 AK094290 AK310967 BC114506 AAI14507 BC114951 AAI14952 GU056176 ADD83109 XM_006720452 XP_006720515 XM_006720453 XP_006720516 XM_011521410 XP_011519712 XM_011521411 XP_011519713 XM_011521412 XP_011519714 XM_011521413 XP_011519715 XM_011521414 XP_011519716 XM_011521415 XP_011519717 XM_011521416 XP_011519718 XM_011521417 XP_011519719 XM_011521418 XP_011519720 XM_011521419 XP_011519721 XM_011521420 XP_011519722 XM_011521421 XP_011519723 NM_014326 NP_055141
Function Calcium/calmodulin-dependent serine/threonine kinase involved in multiple cellular signaling pathways that trigger cell survival, apoptosis, and autophagy. Regulates both type I apoptotic and type II autophagic cell deaths signal, depending on the cellular setting. The former is caspase-dependent, while the latter is caspase-independent and is characterized by the accumulation of autophagic vesicles. Acts as a mediator of anoikis and a suppressor of beta-catenin-dependent anchorage-independent growth of malignant epithelial cells. May play a role in granulocytic maturation.
Subcellular Location Cytoplasm. Cytoplasmic vesicle, autophagosome lumen.
Tissue Specificity Isoform 2 is expressed in embryonic stem cells (at protein level). Isoform 1 is ubiquitously expressed in all tissue types examined with high levels in heart, lung and skeletal muscle. It is expressed abundantly in cells differentiated toward granulocytes and low in undifferentiated, normal and leukemic hematopoietic cells and monocytes/macrophages. {ECO:0000269|PubMed:10376525, ECO:0000269|PubMed:17347302, ECO:0000269|PubMed:21408167}.
Top Pathways Bladder cancer

DAPK2 (C-18) - sc-10084 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

DAPK2 (N-14) - sc-10081 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

DAPK2 (P-24) - sc-130734 from Santa Cruz Biotechnology

Prices $279.00
Sizes 100 µg/ml
Host Rabbit
Clonality Polyclonal
DAPK2 (P-24): sc-130734. Immunoperoxidase staining of formalin-fixed, paraffin-embedded human skeletal muscle tissue showing cytoplasmic localization.

DAPK2 (X15) - sc-100371 from Santa Cruz Biotechnology

Prices $295.00
Sizes 100 µg/ml
Host Mouse
Clonality Monoclonal
DAPK2 (X15): sc-100371. Western blot analysis of DAPK2 expression in A-431 whole cell lysate.

Anti-DAP Kinase 2 (phospho S318) antibody - ab61042 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
1/50-1/100 ab61042 staining phosphorilated DAP Kinase 2 in human skeletal muscle tissue, in the absence or presence of the immunizing phosphopeptide.

Anti-DAP Kinase 2 antibody - ab115538 from Abcam

Prices $376.00
Sizes 50 µg
Host Goat
Clonality Polyclonal
Anti-DAP Kinase 2 antibody (ab115538) at 2 µg/ml + Mouse Brain extracts (in RIPA buffer) at 35 µgdeveloped using the ECL technique

Anti-DAP Kinase 2 antibody - ab8404 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-DAP Kinase 2 antibody (ab8404) at 1 µg/mlLane 1 : A431 whole cell lysateLane 2 : Mouse spleen lysateLane 3 : Rat kidney lysateObserved band size : 42 kDa (why is the actual band size different from the predicted?)

Anti-DAP Kinase 2 antibody - ab203332 from Abcam

Prices $359.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
All lanes : Anti-DAP Kinase 2 antibody (ab203332) at 1/300 dilutionLane 1 : Mouse kidney lysateLane 2 : HepG2 cell lysateSecondaryGoat Anti-Rabbit IgG Antibody (H+L), HRP Conjugated at 1/5000 dilution

Anti-DAP Kinase 2 antibody - ab37995 from Abcam

Prices $378.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
Anti-DAP Kinase 2 antibody (ab37995) + mouse lung tissue lysate

Anti-DAP Kinase 2 antibody - ab61142 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
ab61142 at 1/50 dilution staining human skeletal muscle without (left) and with (right) blocking peptide; paraffin embedded.

Anti-DAP Kinase 2 antibody - ab57175 from Abcam

Prices $376.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal

Anti-DAP Kinase 2 antibody - ab1382 from Abcam

Prices $376.00
Sizes 100 µg
Host Goat
Clonality Polyclonal
DAPK2 staining (2µg/ml) of Mouse Brain extracts (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence

Anti-DAP Kinase 2 antibody [1B10] - ab119035 from Abcam

Prices $384.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 1B10
All lanes : Anti-DAP Kinase 2 antibody [1B10] (ab119035) at 1/2000 dilutionLane 1 : HEK293T cell lysate transfected with pCMV6-ENTRY controlLane 2 : HEK293T cell lysate transfected with pCMV6-ENTRY DAP Kinase 2 cDNALysates/proteins at 5 µg per lane.

Anti-DAP Kinase 2 antibody [EP1633Y] - ab51601 from Abcam

Prices $387.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EP1633Y
Overlay histogram showing HeLa cells stained with ab51601 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab51601, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HeLa cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

Anti-DAP Kinase 2 antibody [EPR1634(2)] - ab111928 from Abcam

Prices $379.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR1634(2)
All lanes : Anti-DAP Kinase 2 antibody [EPR1634(2)] (ab111928) at 1/1000 dilutionLane 1 : Human fetal brain lysateLane 2 : HepG2 cell lysateLane 3 : A431 cell lysateLysates/proteins at 10 µg per lane.

Anti-DAP Kinase 2 antibody, prediluted - ab53994 from Abcam

Prices $370.00
Sizes 7 ml
Host Rabbit
Clonality Polyclonal

Anti-DAPK2 / DAP Kinase 2 Antibody (C-Terminus) IHC-plus™ - LS-B556 from LifeSpan Bioscience

Prices $395.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Anti-DAPK2 antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B556 concentration 5 ug/ml.

Anti-DAPK2 / DAP Kinase 2 Antibody (clone 1B5) IHC-plus™ - LS-B9958 from LifeSpan Bioscience

Prices $395.00
Sizes 50 µl
Host Mouse
Clonality Monoclonal
Clone 1B5
Anti-DAPK2 / DRP1 antibody IHC staining of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B9958 concentration 10 ug/ml.

Anti-DAPK2 / DAP Kinase 2 Antibody (aa359-370) IHC-plus™ - LS-B3593 from LifeSpan Bioscience

Prices $395.00
Sizes 50 µg
Host Goat
Clonality Polyclonal
Anti-DAPK2 antibody IHC of human thyroid. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3593 concentration 2.5 ug/ml.

Anti-DAPK2 / DRP1 Antibody (aa356-370) - LS-C83826 from LifeSpan Bioscience

Host Rabbit
Clonality Polyclonal
DAPK 2 Antibody - Western blot of DAP Kinase 2 in A431 whole cell lysate (lane 1), mouse spleen lysate (lane 2) and rat kidney lysate (lane 3).

Anti-DAPK2 / DAP Kinase 2 Antibody (N-Terminus, clone EP1633Y) - LS-C49962 from LifeSpan Bioscience

Prices $485.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EP1633Y
IHC of paraffin-embedded human liver carcinoma using anti-DAPK2 antibody.

Anti-DAPK2 / DAP Kinase 2 Antibody - LS-C153217 from LifeSpan Bioscience

Prices $545.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-DAPK2 / DAP Kinase 2 Antibody (C-Terminus) - LS-C188959 from LifeSpan Bioscience

Prices $425.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal

Anti-DAPK2 / DAP Kinase 2 Antibody (aa37-65, AP) - LS-C213507 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-DAPK2 / DAP Kinase 2 Antibody (aa37-65, APC) - LS-C213508 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal
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