Anti-DATF1 antibody
| Name | Anti-DATF1 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab92868 |
| Prices | $370.00 |
| Sizes | 100 µg |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Applications | WB ELISA FC |
| Species Reactivities | Human |
| Antigen | Recombinant fragment: ILQVQDETHS ETADQQEAKW RPGDADGTDC TSIGTIEQKS SEDQGIKGRI EKAANPSGKK KLKIFQPVIE APGASKCIGP GCCHVAQPDS VYCSNDCILK, corresponding to amino acids 321-420 of Human DATF1 (AAH14489), with a proprietary tag |
| Description | Mouse Monoclonal |
| Gene | DIDO1 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
![Overlay histogram showing HepG2 cells stained with ab92868 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab92868, 0.1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H+L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_2/6384_ab92868-3-ab92868FC.jpg)
Overlay histogram showing HepG2 cells stained with ab92868 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab92868, 0.1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H+L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
Anti-DATF1 antibody (ab92868) at 5 µg/ml + immunogen (80aa recombinant fragment with a tag of 26kDa) at 0.2 µgSecondaryGoat anti-Mouse IgG HRP conjugated at 1/5000 dilution
Anti-DATF1 antibody (ab92868) at 5 µg/ml + HepG2 cell lysate at 25 µgSecondaryGoat anti-Mouse IgG HRP conjugated at 1/2500 dilution
Product References
Dido3-dependent HDAC6 targeting controls cilium size. - Dido3-dependent HDAC6 targeting controls cilium size.
Sanchez de Diego A, Alonso Guerrero A, Martinez-A C, van Wely KH. Nat Commun. 2014 Mar 25;5:3500.
Proteomic characterization of human coronary thrombus in patients with ST-segment - Proteomic characterization of human coronary thrombus in patients with ST-segment
Alonso-Orgaz S, Moreno-Luna R, Lopez JA, Gil-Dones F, Padial LR, Moreu J, de la Cuesta F, Barderas MG. J Proteomics. 2014 Sep 23;109:368-81.