DIDO1

Gene Symbol DIDO1
Entrez Gene 11083
Alt Symbol BYE1, C20orf158, DATF-1, DATF1, DIDO2, DIDO3, DIO-1, DIO1, dJ885L7.8
Species Human
Gene Type protein-coding
Description death inducer-obliterator 1
Other Description death-associated transcription factor 1|death-inducer obliterator 1
Swissprots Q9UFB6 Q9BW03 Q9NUM8 A8MY65 E1P5I1 Q9H4G6 Q3ZTL8 Q9BQW0 Q4VXS2 Q9NTU8 Q9BTC0 Q96D72 O15043 Q4VXS1 Q9H4G7 B9EH82 Q4VXV9 Q4VXV8 Q3ZTL7
Accessions EAW75321 EAW75322 EAW75323 EAW75324 EAW75325 EAW75326 Q9BTC0 AB002331 BAA20791 AF085876 AI291497 AK002127 BAA92094 AK127121 AL133063 CAB61387 AW206895 AY481571 AAS49898 AY481572 AAS49899 BC000770 AAH00770 BC004237 AAH04237 BC012757 BC014489 AAH14489 BC014615 BC137177 AAI37178 BG677268 BG755041 BG771506 BI830580 BQ045451 BX404270 CD637702 CK300739 CN348577 CN348578 CN370110 CN370112 DA294135 DA506324 DB460917 DQ893020 ABM83946 DQ896265 ABM87264 LK937967 XM_011528504 XP_011526806 XM_011528505 XP_011526807 XM_011528506 XP_011526808 XM_011528507 XP_011526809 XM_011528508 XP_011526810 XM_011528509 XP_011526811 XM_011528510 XP_011526812 NM_001193369 NP_001180298 NM_001193370 NP_001180299 NM_022105 NP_071388 NM_033081 NP_149072 NM_080796 NP_542986 NM_080797 NP_542987
Function Putative transcription factor, weakly pro-apoptotic when overexpressed (By similarity). Tumor suppressor. Required for early embryonic stem cell development. {ECO:0000250, ECO:0000269|PubMed:16127461}.
Subcellular Location Cytoplasm {ECO:0000250}. Nucleus {ECO:0000255|PROSITE-ProRule:PRU00651}. Cytoplasm, cytoskeleton, spindle {ECO:0000269|PubMed:23831028}. Note=Translocates to the nucleus after pro-apoptotic stimuli (By similarity). Translocates to the mitotic spindle upon loss of interaction with H3K4me3 during early mitosis. {ECO:0000250}.
Tissue Specificity Ubiquitous.

DIDO1 Antibody - 3605 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from COS cells, mock transfected or transfected with a construct overexpressing human DIDO1, using DIDO1 Antibody.

Dio-1 (M-300) - sc-11445 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
Dio-1 (M-300): sc-11445. Western blot analysis of Dio-1 expression in Jurkat nuclear extract.

Dio-1 (E-16) - sc-5892 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
Dio-1 (E-16): sc-5892. Western blot analysis of Dio-1 expression in non-transfected: sc-117752 (A) and human Dio-1 transfected: sc-176595 (B) 293T whole cell lysates.

Dio-1 (B-9) - sc-25264 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Dio-1 (B-9): sc-25264. Immunoperoxidase staining of formalin fixed, paraffin-embedded human ovarian cancer showing nuclear staining of tumor cells. Kindly provided by The Swedish Human Protein Atlas (HPA) program.

Anti-DATF1 antibody - ab92868 from Abcam

Prices $370.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Overlay histogram showing HepG2 cells stained with ab92868 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab92868, 0.1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H+L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

Anti-DATF1 antibody - ab168952 from Abcam

Prices $370.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal
All lanes : Anti-DATF1 antibody (ab168952) at 1 µg/mlLane 1 : DATF1 transfected 293T cell lysateLane 2 : Non-transfected 293T cell lysateLysates/proteins at 15 µl per lane.SecondaryGoat anti-mouse IgG at 1/2500 dilution

Anti-DATF1 antibody - ab69804 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-DATF1 antibody (ab69804) at 1/500 dilutionLane 1 : Extracts from 293 cells Lane 2 : Extracts from 293 cells plus immunizing peptideLysates/proteins at 5 µg per lane.

Anti-DATF1 antibody - ab19029 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-DATF1 antibody - C-terminal - ab197947 from Abcam

Prices $370.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue labeling DATF1 with ab197947 at a 1/60 dilution.

Anti-DATF1 / DIDO1 Antibody (N-Terminus) - LS-C48548 from LifeSpan Bioscience

Prices $410.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-DATF1 / DIDO1 Antibody (N-Terminus) - LS-C30278 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Fetal Thymus Lysate

Anti-DATF1 / DIDO1 Antibody (aa161-210) - LS-C119545 from LifeSpan Bioscience

Prices $315.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Western blot of extracts from 293 cells, using DIDO1 Antibody. The lane on the right is treated with the synthesized peptide.

Anti-DATF1 / DIDO1 Antibody - LS-C307864 from LifeSpan Bioscience

Prices $455.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal

Anti-DATF1 / DIDO1 Antibody (aa480-529) - LS-C80267 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Transfected 293T cell lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%.

Anti-DATF1 / DIDO1 Antibody - LS-C335672 from LifeSpan Bioscience

Prices $255.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts of various cell lines, using DIDO1 antibody.

Anti-DATF1 / DIDO1 Antibody (aa300-350) - LS-C287079 from LifeSpan Bioscience

Prices $375.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Detection of human DATF by Western Blot. Samples: Nuclear extract (5 and 20 ug) from HeLa cells. Antibody: Affinity purified rabbit anti-DATF1 antibody used at 0.01 ug/ml. Detection: Chemiluminescence with an exposure time of 15 minutes.

Anti-DATF1 / DIDO1 Antibody (N-Terminus) - LS-C345382 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Western blot of DIDO1 Antibody with human Jurkat Whole Cell lysate.

Anti-DATF1 / DIDO1 Antibody (clone 3B1) - LS-C139237 from LifeSpan Bioscience

Prices $355.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 3B1
DIDO1 monoclonal antibody (M04), clone 3B1. Western blot of DIDO1 expression in HepG2.

Anti-DATF1 / DIDO1 Antibody (aa72-121) IHC-plus™ - LS-B1519 from LifeSpan Bioscience

Prices $450.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Anti-DIDO1 / DATF1 antibody IHC of human thyroid. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1519 concentration 5 ug/ml.

Anti-DATF1 / DIDO1 Antibody (Ser186) - LS-C177043 from LifeSpan Bioscience

Prices $345.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
IHC of DATF1 / DIDO1 (S186) pAb in paraffin-embedded human colon carcinoma tissue.

DIDO1 Antibody - NBP1-19706 from Novus Biologicals

Prices $349.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Western Blot: DIDO1 Antibody [NBP1-19706] - Western blot (WB) analysis of DIDO-1 (S186) pAb at 1:500 dilution

DIDO1 Antibody (734823) - MAB6947 from Novus Biologicals

Prices $99.00, $249.00
Sizes 25 µg, 100 µg
Host Mouse
Clonality Monoclonal
Clone 734823
Western blot shows lysates of Raji human Burkitt's lymphoma cell line. Gels were loaded with 30 µg of whole cell lysate (WCL), 20 µg of cytoplasmic (Cyto), and 10 µg of nuclear (Nuc) extracts. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human DIDO1 Monoclonal Antibody (Catalog # MAB6947) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for DIDO1 at approximately 280 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

DIDO1 Antibody - AF6947 from Novus Biologicals

Prices $99.00, $359.00
Sizes 25 µg, 100 µg
Host Sheep
Clonality Polyclonal
Western blot shows lysates of Raji human Burkitt's lymphoma cell line. Gels were loaded with 30 µg of whole cell lysate (WCL), 20 µg of cytoplasmic (Cyto), and 10 µg of nuclear extracts (Nuc). PVDF membrane was probed with 0.2 µg/mL of Sheep Anti-Human DIDO1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6947) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for DIDO1 at approximately 270 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

DIDO1 Antibody - NBP2-33269 from Novus Biologicals

Prices $419.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Western Blot: DIDO1 Antibody [NBP2-33269] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10Lane 2: Human cell line RT-4

DIDO1 Antibody - H00011083-B01P from Novus Biologicals

Prices $359.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal
Western Blot: DIDO1 Antibody [H00011083-B01P] - Analysis of DIDO1 expression in transfected 293T cell line by DIDO1 polyclonal antibody. Lane 1: DIDO1 transfected lysate(59.84 KDa). Lane 2: Non-transfected lysate.
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