Anti-Dcp1a antibody [EPR13822] (ab183709) at 1/10000 dilution + 293T cell lysate at 20 µgSecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
All lanes : Anti-Dcp1a antibody [EPR13822] (ab183709) at 1/2000 dilutionLane 1 : HeLa cell lysateLane 2 : HepG2 cell lysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling Dcp1a with ab183709 at 1/250 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.
Immunofluorescent analysis of 4% paraformaldehyde-fixed HepG2 cells labeling Dcp1a with ab183709 at 1/250 dilution, followed by Goat anti rabbit IgG (Alexa Fluor® 488) secondary antibody at 1/200 dilution (green). Counter stained with Dapi (blue).
Flow cytometric analysis of 2% paraformaldehyde-fixed HeLa cells labeling Dcp1a with ab183709 at 1/150 dilution (red) compared to a Rabbit monoclonal IgG isotype control (green), followed by Goat anti rabbit IgG (FITC) secondary antibody at 1/150 dilution.