All lanes : Anti-DCUN1D1 antibody [EPR13492] (ab181233) at 1/20000 dilutionLane 1 : A549 cell lysateLane 2 : HT29 cell lysateLane 3 : HepG2 cell lysateLane 4 : HeLa cell lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Immunoprecipitation analysis of HeLa cells labeling DCUN1D1 using ab181233 at 1/50 dilution. A Goat Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG was used as secondary antibody at a 1/1500 dilution.