Anti-DCXR antibody [4G4AF5]
| Name | Anti-DCXR antibody [4G4AF5] |
|---|---|
| Supplier | Abcam |
| Catalog | ab110283 |
| Prices | $359.00 |
| Sizes | 100 µg |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Clone | 4G4AF5 |
| Applications | WB ICC/IF ICC/IF ELISA FC IP |
| Species Reactivities | Human |
| Antigen | Human liver mitochondria |
| Description | Mouse Monoclonal |
| Gene | DCXR |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
![Anti-DCXR antibody [4G4AF5] (ab110283) at 1 µg/ml + Human liver mitochondria at 20 µgSecondaryGoat anti-mouse conjugated to AP](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_2/6866_DCXR-Primary-antibodies-ab110283-4.gif)
Anti-DCXR antibody [4G4AF5] (ab110283) at 1 µg/ml + Human liver mitochondria at 20 µgSecondaryGoat anti-mouse conjugated to AP
Immunocytochemistry image of ab110283 stained Human HDFn cells. The cells were paraformaldehyde fixed (4%, 20 min) and Triton X-100 permeabilized (0.1%, 15min). The cells were incubated with ab110283 at 4 µg/ml for 2h at room temperature or over night at 4°C. The secondary antibody was (green) AlexaFluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. 10% Goat serum was used as the blocking agent for all blocking steps. DAPI was used to stain the cell nuclei (blue). Target protein locates mainly in the cell membrane.
HL-60 cells were stained with 1 µg/mL ab110283 (blue) or an equal amount of an isotype control antibody (red) and analyzed by flow cytometry.