Anti-DDAH1 antibody

• Supplier: Abcam
• Catalog: ab2231
• Prices: $398.00
• Sizes: 100 µg
• Host: Goat
• Clonality: Polyclonal
• Isotype: IgG
• Applications: IHC-P ICC/IF ICC/IF IHC-F WB
• Species Reactivities: Rat, Human, Bovine
• Antigen: Synthetic peptide: TCCSVLINKKVDS , corresponding to C terminal amino acids 272-284 of Human DDAH1
• Blocking Peptide: Human DDAH1 peptide
• Description: Goat Polyclonal
• Gene: DDAH1
• Conjugate: Unconjugated

Product images

Anti-DDAH1 antibody (ab2231) at 0.10000000149 µg/ml + Human kidney lysate at 35 µg

ICC/IF image of ab2231 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal donkey serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab2231, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 donkey anti-goat IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

IHC image of ab2231 staining in human normal kidney formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab2231, 10µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Product References

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Jung CS, Wispel C, Zweckberger K, Beynon C, Hertle D, Sakowitz OW, Unterberg AW. Int J Mol Sci. 2014 Mar 6;15(3):4088-103.

FXR agonist INT-747 upregulates DDAH expression and enhances insulin sensitivity - FXR agonist INT-747 upregulates DDAH expression and enhances insulin sensitivity

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Ghebremariam YT, Erlanson DA, Yamada K, Cooke JP. J Biomol Screen. 2012 Jun;17(5):651-61.

Regulation of myometrial contractivity during pregnancy in the rat: potential - Regulation of myometrial contractivity during pregnancy in the rat: potential

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Role of asymmetric dimethylarginine in vascular injury in transgenic mice - Role of asymmetric dimethylarginine in vascular injury in transgenic mice

Hasegawa K, Wakino S, Tatematsu S, Yoshioka K, Homma K, Sugano N, Kimoto M, Hayashi K, Itoh H. Circ Res. 2007 Jul 20;101(2):e2-10. Epub 2007 Jun 29.