DDAH1

Gene Symbol DDAH1
Entrez Gene 23576
Alt Symbol DDAH, HEL-S-16
Species Human
Gene Type protein-coding
Description dimethylarginine dimethylaminohydrolase 1
Other Description DDAH-1|DDAHI|N(G),N(G)-dimethylarginine dimethylaminohydrolase 1|NG, NG-dimethylarginine dimethylaminohydrolase|dimethylargininase-1|epididymis secretory protein Li 16
Swissprots Q86XK5 O94760 Q5HYC8
Accessions EAW73199 EAW73200 EAW73201 EAW73202 O94760 AK024917 AK026093 AK055626 AK098405 AK294752 BAG57891 AK302531 BAG63803 AK304876 BAG65613 AK309225 AK312432 BAG35341 AW051386 BC033680 AAH33680 BC036432 BC043235 AAH43235 BX648145 CAI45988 DC324361 EU794605 ACJ13659 XM_005270707 XP_005270764 XM_005270709 XP_005270766 XM_005270710 XP_005270767 XM_006710544 XP_006710607 XM_011541158 XP_011539460 NM_001134445 NP_001127917 NM_012137 NP_036269
Function Hydrolyzes N(G),N(G)-dimethyl-L-arginine (ADMA) and N(G)-monomethyl-L-arginine (MMA) which act as inhibitors of NOS. Has therefore a role in the regulation of nitric oxide generation.
Tissue Specificity Detected in brain, liver, kidney and pancreas, and at low levels in skeletal muscle. {ECO:0000269|PubMed:10493931}.

DDAH I (C-4) - sc-271337 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
DDAH I (C-4): sc-271337. Western blot analysis of DDAH I expression in KNRK whole cell lysate.

DDAH I (C-19) - sc-26068 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
DDAH I (C-19): sc-26068. Western blot analysis of DDAH I expression in non-transfected: sc-117752 (A) and mouse DDAH I transfected: sc-119696 (B) 293T whole cell lysates.

DDAH I (H-70) - sc-292503 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
DDAH I (H-70): sc-292503. Western blot analysis of DDAH I expression in mouse brain tissue extract.

Anti-DDAH1 antibody - ab2231 from Abcam

Prices $398.00
Sizes 100 µg
Host Goat
Clonality Polyclonal
Anti-DDAH1 antibody (ab2231) at 0.10000000149 µg/ml + Human kidney lysate at 35 µg

Anti-DDAH1 antibody - ab127579 from Abcam

Prices $376.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal
All lanes : Anti-DDAH1 antibody (ab127579) at 1 µg/mlLane 1 : Lung (Human) Whole Cell Lysate - fetal normal tissue (ab30282)Lane 2 : Liver (Human) Tissue Lysate - adult normal tissue (ab29889)Lane 3 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell LysateLane 4 : Kidney (Human) Tissue Lysate - adult normal tissue (ab30203)Lane 5 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate Lane 6 : Pancreas (Human) Tissue Lysate - adult normal tissue (ab29816)Lysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.

Anti-DDAH1 antibody - ab82908 from Abcam

Prices $384.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal

Anti-DDAH1 antibody - ab108088 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-DDAH1 antibody (ab108088) at 1 µg/mlLane 1 : Lung (Human) Whole Cell Lysate - fetal normal tissue (ab30282)Lane 2 : Liver (Human) Tissue Lysate - adult normal tissue (ab29889)Lane 3 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell LysateLane 4 : Kidney (Human) Tissue Lysate - adult normal tissue (ab30203)Lane 5 : HEK293 (Human embryonic kidney cell line) Whole Cell LysateLane 6 : Brain (Human) Tissue Lysate - adult normal tissue (ab29466)Lane 7 : Pancreas (Human) Tissue Lysate - adult normal tissue (ab29816)Lysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.

Anti-DDAH1 antibody - ab97756 from Abcam

Prices $376.00
Sizes 50 µl
Host Rabbit
Clonality Polyclonal
Anti-DDAH1 antibody (ab97756) at 1/1000 dilution + Raji whole cell lysate at 30 µg

Anti-DDAH1 antibody - ab167500 from Abcam

Prices $376.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal
All lanes : Anti-DDAH1 antibody (ab167500)Lane 1 : DDAH1 transfected 293T cell lysate Lane 2 : Non-transfected lysate 293T cell lysate Lysates/proteins at 15 µl per lane.

Anti-DDAH1 antibody - ab167519 from Abcam

Prices $376.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal
All lanes : Anti-DDAH1 antibody (ab167519) at 1 µg/mlLane 1 : DDAH1 transfected 293T cell lysateLane 2 : Non-transfected 293T cell lysateLysates/proteins at 15 µl per lane.

Anti-DDAH1 antibody [EPR13921] - ab181859 from Abcam

Prices $384.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR13921
Western blot analysis of 293 cell lysate immunoprecipitated using ab181859 at 1/40 dilution. Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate secondary antibody was used at 1/1000 dilution.

Anti-DDAH1 antibody [EPR13922] - ab180599 from Abcam

Prices $384.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR13922
All lanes : Anti-DDAH1 antibody [EPR13922] (ab180599) at 1/20000 dilutionLane 1 : Human fetal brain lysate at 20 µgLane 2 : Human fetal liver lysate at 20 µgLane 3 : Human fetal kidney lysate at 20 µgLane 4 : 293 lysate at 10 µgSecondaryGoat Anti-Rabbit IgG (H+L) Peroxidase conjugated at 1/1000 dilutionObserved band size : 37 kDa (why is the actual band size different from the predicted?)

Anti-DDAH1 Antibody (aa20-215, FITC) - LS-C303136 from LifeSpan Bioscience

Prices $455.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-DDAH1 Antibody (aa20-215, Biotin) - LS-C300198 from LifeSpan Bioscience

Prices $425.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-DDAH1 Antibody - LS-C307198 from LifeSpan Bioscience

Prices $455.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal

Anti-DDAH1 Antibody (aa181-286) - LS-C307199 from LifeSpan Bioscience

Prices $455.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal

Anti-DDAH1 Antibody - LS-C310100 from LifeSpan Bioscience

Prices $475.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-DDAH1 Antibody (aa273-285) - LS-C54710 from LifeSpan Bioscience

Prices $395.00
Sizes 100 µg
Host Goat
Clonality Polyclonal
Antibody staining (0.1 ug/ml) of human kidney lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by Western blot of chemiluminescence.

Anti-DDAH1 Antibody (aa20-215) - LS-C293691 from LifeSpan Bioscience

Prices $405.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Western blot of recombinant DDAH / DDAH1.

Anti-DDAH1 Antibody (aa223-285) - LS-C185642 from LifeSpan Bioscience

Prices $335.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Immunofluorescence of methanol-fixed HCT116 using DDAH1 antibody at 1:500 dilution.

Anti-DDAH1 Antibody (aa108-157) IHC-plus™ - LS-B5857 from LifeSpan Bioscience

Prices $450.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Anti-DDAH1 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B5857 concentration 2.5 ug/ml.

Anti-DDAH1 Antibody (aa221-250) - LS-C167312 from LifeSpan Bioscience

Prices $295.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
Formalin-fixed and paraffin-embedded mouse kidney reacted with DDAH1 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Anti-DDAH1 Antibody (aa108-157) IHC-plus™ - LS-B5858 from LifeSpan Bioscience

Prices $450.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Anti-DDAH1 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B5858 concentration 2.5 ug/ml.

Dimethylarginine Dimethylaminohydrolase 1/DDAH1 Antibody - AF6530 from Novus Biologicals

Prices $99.00, $359.00
Sizes 25 µg, 100 µg
Host Sheep
Clonality Polyclonal
Dimethylarginine Dimethylaminohydrolase 1/DDAH1 was detected in immersion fixed paraffin-embedded sections of human brain using Sheep Anti-Human Dimethylarginine Dimethylaminohydrolase 1/DDAH1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6530) at 3 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counter­stained with hematoxylin (blue). Specific staining was localized to neuronal cell bodies and processes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Dimethylarginine Dimethylaminohydrolase 1/DDAH1 Antibody - NBP1-85273 from Novus Biologicals

Prices $419.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Western Blot: Dimethylarginine Dimethylaminohydrolase 1/DDAH1 Antibody [NBP1-85273] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.
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