Western blot analysis of 293 cell lysate immunoprecipitated using ab181859 at 1/40 dilution. Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate secondary antibody was used at 1/1000 dilution.
Flow cytometric analysis of 2% paraformaldehyde-fixed 293 cells labeling DDAH1 with ab181859 at 1/30 dilution (red), compared to a Rabbit monoclonal IgG isotype control (green), followed by Goat anti rabbit IgG (FITC) secondary antibody at 1/150 dilution.
Immunohistochemical analysis of paraffin-embedded Human hepatocellular carcinoma tissue labeling DDAH1 with ab181859 at 1/50 dilution, followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.
All lanes : Anti-DDAH1 antibody [EPR13921] (ab181859) at 1/5000 dilutionLane 1 : 293 cell lysateLane 2 : HepG2 cell lysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution