Anti-DDAH1 antibody [EPR13922]

• Supplier: Abcam
• Catalog: ab180599
• Prices: $384.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: EPR13922
• Applications: WB IHC-P ICC/IF ICC/IF IP
• Species Reactivities: Mouse, Rat, Human, Bovine
• Antigen: Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human DDAH1 aa 200 to the C-terminus
• Description: Rabbit Monoclonal
• Gene: DDAH1
• Conjugate: Unconjugated

Product images

All lanes : Anti-DDAH1 antibody [EPR13922] (ab180599) at 1/20000 dilutionLane 1 : Human fetal brain lysate at 20 µgLane 2 : Human fetal liver lysate at 20 µgLane 3 : Human fetal kidney lysate at 20 µgLane 4 : 293 lysate at 10 µgSecondaryGoat Anti-Rabbit IgG (H+L) Peroxidase conjugated at 1/1000 dilutionObserved band size : 37 kDa (why is the actual band size different from the predicted?)

Immunohistochemical analysis of paraffin embedded Human ovarian carcinoma tissue labeling DDAH1 with ab180599 at a 1/50 dilution. Prediluted HRP Polymer for Rabbit IgG secondary used. Counterstained with Hematoxylin.

Immunofluorescence analysis of 293 cells (fixative 4% paraformaldehyde) labeling DDAH1 with ab180599 at a 1/100 dilution (left image), and counterstained with Dapi (right image). Goat anti rabbit IgG (Dylight 555) secondary used at a 1/200 diution.

Immunoprecipitation analysis of Human fetal kidney lysate labeling DDAH1 with ab180599 at a 1/40 dilution. Goat Anti-Rabbit IgG (H+L) Peroxidase conjugated secondary used at a 1/1000 dilution.