Anti-DDB2 antibody [2246C4a]
| Name | Anti-DDB2 antibody [2246C4a] |
|---|---|
| Supplier | Abcam |
| Catalog | ab51017 |
| Prices | $400.00 |
| Sizes | 50 µg |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Clone | 2246C4a |
| Applications | IHC-P ICC/IF ICC/IF WB DB FC |
| Species Reactivities | Human |
| Antigen | Recombinant fragment (N-terminal) Human |
| Description | Mouse Monoclonal |
| Gene | DDB2 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
![Anti-DDB2 antibody [2246C4a] (ab51017) at 1/50 dilution + HeLa whole cell lysate at 50 µgSecondaryMouse IgG antibody at 1/2500 dilutiondeveloped using the ECL technique](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_2/6941_ddb2.jpg)
Anti-DDB2 antibody [2246C4a] (ab51017) at 1/50 dilution + HeLa whole cell lysate at 50 µgSecondaryMouse IgG antibody at 1/2500 dilutiondeveloped using the ECL technique
IHC image of ab51017 staining in human normal cervical carcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab51017, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
ICC/IF image of ab51017 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab51017, 10µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
![Overlay histogram showing HeLa cells stained with ab51017 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab51017, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells ) used under the same conditions. Acquisition of >5,000 events was performed.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_2/6944_DDB2-Primary-antibodies-ab51017-4.jpg)
Overlay histogram showing HeLa cells stained with ab51017 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab51017, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells ) used under the same conditions. Acquisition of >5,000 events was performed.
Product References
A novel DDB2-ATM feedback loop regulates human cytomegalovirus replication. - A novel DDB2-ATM feedback loop regulates human cytomegalovirus replication.
E X, Savidis G, Chin CR, Wang S, Lu S, Brass AL, Kowalik TF. J Virol. 2014 Feb;88(4):2279-90.
Low-dose formaldehyde delays DNA damage recognition and DNA excision repair in - Low-dose formaldehyde delays DNA damage recognition and DNA excision repair in
Luch A, Frey FC, Meier R, Fei J, Naegeli H. PLoS One. 2014 Apr 10;9(4):e94149.
DDB2 suppresses tumorigenicity by limiting the cancer stem cell population in - DDB2 suppresses tumorigenicity by limiting the cancer stem cell population in
Han C, Zhao R, Liu X, Srivastava A, Gong L, Mao H, Qu M, Zhao W, Yu J, Wang QE. Mol Cancer Res. 2014 May;12(5):784-94.
p38 MAPK- and Akt-mediated p300 phosphorylation regulates its degradation to - p38 MAPK- and Akt-mediated p300 phosphorylation regulates its degradation to
Wang QE, Han C, Zhao R, Wani G, Zhu Q, Gong L, Battu A, Racoma I, Sharma N, Wani AA. Nucleic Acids Res. 2013 Feb 1;41(3):1722-33.
The NR4A2 nuclear receptor is recruited to novel nuclear foci in response to UV - The NR4A2 nuclear receptor is recruited to novel nuclear foci in response to UV
Jagirdar K, Yin K, Harrison M, Lim W, Muscat GE, Sturm RA, Smith AG. PLoS One. 2013 Nov 6;8(11):e78075.
Protein arginine methyltransferase 7 regulates cellular response to DNA damage by - Protein arginine methyltransferase 7 regulates cellular response to DNA damage by
Karkhanis V, Wang L, Tae S, Hu YJ, Imbalzano AN, Sif S. J Biol Chem. 2012 Aug 24;287(35):29801-14.