All lanes : Anti-DDB2 antibody [EPR9811] (ab181136) at 1/10000 dilutionLane 1 : HeLa cell lysateLane 2 : Raji cell lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Immunohistochemical analysis of paraffin-embedded Human breast tissue labeling DDB2 with ab181136 at 1/100 dilution, followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.
Immunohistochemical analysis of paraffin-embedded Human cervical carcinoma tissue labeling DDB2 with ab181136 at 1/100 dilution, followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.
Immunofluorescent analysis of acetone-fixed HeLa cells labeling DDB2 with ab181136 at 1/100 dilution followed by Goat anti rabbit IgG (Alexa Fluor® 488) secondary antibody at 1/200 dilution (left panel). Counter stained with Dapi (right panel).
Flow cytometric analysis of 2% paraformaldehyde-fixed K562 cells labeling DDB2 with ab181136 at 1/10 dilution (red) compared to a Rabbit monoclonal IgG Isotype control (green), followed by Goat anti rabbit IgG (FITC) at 1/150 dilution.