Anti-DDIT3 antibody [9C8]

• Supplier: Abcam
• Catalog: ab11419
• Prices: $403.00
• Sizes: 50 µg
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG2b
• Clone: 9C8
• Applications: WB IP ICC/IF ICC/IF ICC/IF IHC-F FC IHC-P
• Species Reactivities: Mouse, Rat, Human
• Antigen: Other Immunogen Type corresponding to DDIT3
• Description: Mouse Monoclonal
• Gene: DDIT3
• Conjugate: Unconjugated

Product images

ab11419 staining DDIT3 in SKNSH cells treated with deltamethrin (ab141019), by ICC/IF. Increase of DDIT3 expression correlates with increased concentration of deltamethrin, as described in literature.The cells were incubated at 37°C for 48 hours in media containing different concentrations of ab141019 (deltamethrin) in DMSO, fixed with 100% methanol for 5 minutes at -20°C and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab11419 (10 μg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 anti-mouse polyclonal antibody (ab96879) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.

All lanes : Anti-DDIT3 antibody [9C8] (ab11419) at 1/1000 dilutionLane 1 : Mouse hepatocyte whole cell lysateLane 2 : Mouse hepatocyte whole cell lysateLysates/proteins at 20 µg per lane.SecondaryHRP-conjugated goat anti-mouse IgG polyclonal at 1/10000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.

All lanes : Anti-DDIT3 antibody [9C8] (ab11419) at 1/500 dilution (in TBST + 2.5% milk for 16 hours at 4°C)Lane 1 : Whole cell lystate of Mouse 3T3 cellsLane 2 : Whole cell lystate of Mouse 3T3 cells treated with tunicamycin for 24 hoursLysates/proteins at 50 µg per lane.SecondaryAn HRP-conjugated Goat anti-mouse IgG monoclonal at 1/2000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.

ab11419 staining GADD153 in human HeLa cells by Immunocytochemistry/ Immunofluorescence. The cells were fixed in paraformaldehyde, permeabilised in 0.5% Triton X and then blocked using 3% BSA for 1 hour at 24°C. Samples were then incubated with primary antibody at a 1/100 dilution for 1 hour at 24°C. The secondary antibody used was a goat anti-mouse IgG conjugated to Alexa Fluor® 488 (green) used at a 1/1000 dilution.HeLa cells treated with 20uM Tunicamycin for 15 minutes before fixation.See Abreview

Overlay histogram showing HeLa cells stained with ab11419 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab11419, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b  (ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

Product References

HERG K+ channel-dependent apoptosis and cell cycle arrest in human glioblastoma - HERG K+ channel-dependent apoptosis and cell cycle arrest in human glioblastoma

Staudacher I, Jehle J, Staudacher K, Pledl HW, Lemke D, Schweizer PA, Becker R, Katus HA, Thomas D. PLoS One. 2014 Feb 6;9(2):e88164.

beta-cell dysfunction due to increased ER stress in a stem cell model of Wolfram - beta-cell dysfunction due to increased ER stress in a stem cell model of Wolfram

Shang L, Hua H, Foo K, Martinez H, Watanabe K, Zimmer M, Kahler DJ, Freeby M, Chung W, LeDuc C, Goland R, Leibel RL, Egli D. Diabetes. 2014 Mar;63(3):923-33.

Zebrafish transgenic line huORFZ is an effective living bioindicator for - Zebrafish transgenic line huORFZ is an effective living bioindicator for

Lee HC, Lu PN, Huang HL, Chu C, Li HP, Tsai HJ. PLoS One. 2014 Mar 3;9(3):e90160.

Isoflurane induces endoplasmic reticulum stress and caspase activation through - Isoflurane induces endoplasmic reticulum stress and caspase activation through

Wang H, Dong Y, Zhang J, Xu Z, Wang G, Swain CA, Zhang Y, Xie Z. Br J Anaesth. 2014 Oct;113(4):695-707.

Lack of mitochondrial toxicity of darunavir, raltegravir and rilpivirine in - Lack of mitochondrial toxicity of darunavir, raltegravir and rilpivirine in

Blas-Garcia A, Polo M, Alegre F, Funes HA, Martinez E, Apostolova N, Esplugues JV. J Antimicrob Chemother. 2014 Nov;69(11):2995-3000.

Bortezomib-induced unfolded protein response increases oncolytic HSV-1 - Bortezomib-induced unfolded protein response increases oncolytic HSV-1

Yoo JY, Hurwitz BS, Bolyard C, Yu JG, Zhang J, Selvendiran K, Rath KS, He S, Bailey Z, Eaves D, Cripe TP, Parris DS, Caligiuri MA, Yu J, Old M, Kaur B. Clin Cancer Res. 2014 Jul 15;20(14):3787-98.

The endoplasmic reticulum stress marker CHOP predicts survival in malignant - The endoplasmic reticulum stress marker CHOP predicts survival in malignant

Dalton LE, Clarke HJ, Knight J, Lawson MH, Wason J, Lomas DA, Howat WJ, Rintoul RC, Rassl DM, Marciniak SJ. Br J Cancer. 2013 Apr 2;108(6):1340-7.

NOX2 deficiency attenuates markers of adiposopathy and brain injury induced by - NOX2 deficiency attenuates markers of adiposopathy and brain injury induced by

Pepping JK, Freeman LR, Gupta S, Keller JN, Bruce-Keller AJ. Am J Physiol Endocrinol Metab. 2013 Feb 15;304(4):E392-404. doi:

Allelic heterogeneity contributes to variability in ocular dysgenesis, myopathy - Allelic heterogeneity contributes to variability in ocular dysgenesis, myopathy

Kuo DS, Labelle-Dumais C, Mao M, Jeanne M, Kauffman WB, Allen J, Favor J, Gould DB. Hum Mol Genet. 2014 Apr 1;23(7):1709-22.

Endoplasmic reticulum stress mediates house dust mite-induced airway epithelial - Endoplasmic reticulum stress mediates house dust mite-induced airway epithelial

Hoffman SM, Tully JE, Nolin JD, Lahue KG, Goldman DH, Daphtary N, Aliyeva M, Irvin CG, Dixon AE, Poynter ME, Anathy V. Respir Res. 2013 Dec 24;14:141.