DDIT3

Gene Symbol DDIT3
Entrez Gene 1649
Alt Symbol CEBPZ, CHOP, CHOP-10, CHOP10, GADD153
Species Human
Gene Type protein-coding
Description DNA-damage-inducible transcript 3
Other Description C/EBP zeta|CCAAT/enhancer-binding protein homologous protein|DDIT-3|DNA damage-inducible transcript 3 protein|c/EBP-homologous protein 10|growth arrest and DNA damage-inducible protein GADD153
Swissprots F8VS99 P35638
Accessions AAW56077 EAW97021 EAW97022 EAW97023 P35638 AA054097 AA476561 AI658803 AK316581 BAG38169 AV729744 AY461580 BC003637 AAH03637 BC064556 BC107859 BM464290 BT006691 AAP35337 BU664077 CN482428 EU831488 ACE87175 EU831575 ACE86494 S40706 AAB22646 XM_011538006 XP_011536308 NM_001195053 NP_001181982 NM_001195054 NP_001181983 NM_001195055 NP_001181984 NM_001195056 NP_001181985 NM_001195057 NP_001181986 NM_004083 NP_004074
Function Multifunctional transcription factor in ER stress response. Plays an essential role in the response to a wide variety of cell stresses and induces cell cycle arrest and apoptosis in response to ER stress. Plays a dual role both as an inhibitor of CCAAT/enhancer-binding protein (C/EBP) function and as an activator of other genes. Acts as a dominant-negative regulator of C/EBP-induced transcription: dimerizes with members of the C/EBP family, impairs their association with C/EBP binding sites in the promoter regions, and inhibits the expression of C/EBP regulated genes. Positively regulates the transcription of TRIB3, IL6, IL8, IL23, TNFRSF10B/DR5, PPP1R15A/GADD34, BBC3/PUMA, BCL2L11/BIM and ERO1L. Negatively regulates; expression of BCL2 and MYOD1, ATF4-dependent transcriptional activation of asparagine synthetase (ASNS), CEBPA-dependent transcriptional activation of hepcidin (HAMP) and CEBPB-mediated expression of peroxisome proliferator-activated receptor gamma (PPARG). Inhibits the c
Subcellular Location Cytoplasm. Nucleus. Note=Present in the cytoplasm under non-stressed conditions and ER stress leads to its nuclear accumulation.
Top Pathways MAPK signaling pathway, Protein processing in endoplasmic reticulum, Non-alcoholic fatty liver disease (NAFLD), Transcriptional misregulation in cancer

CHOP (L63F7) Mouse mAb - 2895 from Cell Signaling Technology

Prices $99.00, $246.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Mouse
Clonality Monoclonal
Clone L63F7
Western blot analysis of extracts from C6 and A-204 cells, untreated or treated with thapsigargin (300 nM, 2 hours) or tunicamycin (24 μg/ml, 2 hours), using CHOP (L63F7) Mouse mAb.

CHOP (D46F1) Rabbit mAb - 5554 from Cell Signaling Technology

Prices $99.00, $246.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D46F1
Western blot analysis of extracts from C2C12 cells, untreated or tunicamycin-treated (2 μg/ml, 8 hr), using CHOP (D46F1) Rabbit mAb (upper) or β-Actin Antibody #4967 (lower).

GADD 153 (B-3) - sc-7351 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
GADD 153 (B-3): sc-7351. Western blot analysis of GADD 153 expression in cell lysates prepared from control (A) and LPS/PMA-treated (B) RAW 264.7 cells.

GADD 153 (F-168) - sc-575 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
GADD 153 (F-168): sc-575. Immunoperoxidase staining of formalin-fixed, paraffin-embedded human breast tumor showing nuclear staining.

GADD 153 (H-5) - sc-166682 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
GADD 153 (H-5): sc-166682. Western blot analysis of full length mouse recombinant GADD 153 fusion protein.

GADD 153 (R-20) - sc-793 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
GADD 153 (R-20): sc-793. Western blot analysis of mouse recombinant GADD 153 fusion protein.

GADD 153 (9C8) - sc-56107 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
GADD 153 (9C8): sc-56107. Western blot analysis of GADD 153 expression in non-transfected: sc-117752 (A) and mouse GADD 153 transfected: sc-120383 (B) 293T whole cell lysates.

GADD 153 (6D313) - sc-71136 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
GADD 153 (6D313): sc-71136. Western blot analysis of GADD 153 expression in non-transfected: sc-117752 (A) and mouse GADD 153 transfected: sc-120383 (B) 293T whole cell lysates.

Anti-DDIT3 (phospho S30) antibody - ab63392 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Immunofluorescence analysis of A549 cells, using ab63392 at 1:500 dilution.Left image untreated.Right image treated with phospho peptide.

Anti-DDIT3 (phospho S30) antibody - ab195713 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-DDIT3 (phospho S30) antibody (ab195713) at 1/500 dilutionLane 1 : PMA treated Jurkat whole cell lysate with antigen-specific peptideLane 2 : PMA treated Jurkat whole cell lysateLysates/proteins at 12.5 µg per lane.

Anti-DDIT3 (phospho S30) antibody - ab194533 from Abcam

Prices $370.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
All lanes : Anti-DDIT3 (phospho S30) antibody (ab194533) at 1/500 dilutionLane 1 : Jurkat cell extract, treated with PMALane 2 : Jurkat cell extract, treated with PMA with antigen-specific peptide

Anti-DDIT3 antibody - ab10444 from Abcam

Prices $372.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-DDIT3 antibody - ab27539 from Abcam

Host Rabbit
Clonality Polyclonal
Staining of human breast carcinoma using ab27539

Anti-DDIT3 antibody - ab53081 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-DDIT3 antibody (ab53081) at 1/500 dilutionLane 1 : LOVO cell extract (treated with 10% serum for 15 mins.) with no blocking peptideLane 2 : LOVO cell extract (treated with 10% serum for 15 mins.) with blocking peptide (ab53081 immunogen)

Anti-DDIT3 antibody - ab171894 from Abcam

Prices $376.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal
All lanes : Anti-DDIT3 antibody (ab171894) at 1 µg/mlLane 1 : DDIT3 transfected 293T cell line lysateLane 2 : Non-transfected 293T cell line lysateLysates/proteins at 15 µl per lane.

Anti-DDIT3 antibody [9C8] - ab11419 from Abcam

Prices $403.00
Sizes 50 µg
Host Mouse
Clonality Monoclonal
Clone 9C8
ab11419 staining DDIT3 in SKNSH cells treated with deltamethrin (ab141019), by ICC/IF. Increase of DDIT3 expression correlates with increased concentration of deltamethrin, as described in literature.The cells were incubated at 37°C for 48 hours in media containing different concentrations of ab141019 (deltamethrin) in DMSO, fixed with 100% methanol for 5 minutes at -20°C and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab11419 (10 μg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 anti-mouse polyclonal antibody (ab96879) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.

Anti-DDIT3 antibody [EPR4943(2)] - C-terminal - ab179823 from Abcam

Prices $388.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR4943(2)
Immunohistochemical analysis of paraffin-embedded Human pancreas tissue labeling DDIT3 using ab179823 at 1/50 dilution.

Anti-DDIT3 antibody, prediluted - ab27540 from Abcam

Prices $376.00
Sizes 7 ml
Host Rabbit
Clonality Polyclonal
Ab27540, prediluted, staining formalin fixed, paraffin embedded human breast carcinoma sections by Immunohistochemistry.

Anti-DDIT3 antibody - C-terminal - ab170379 from Abcam

Host Rabbit
Clonality Polyclonal
Anti-DDIT3 antibody - C-terminal (ab170379) at 1/100 dilution + Mouse testis tissue lysate at 35 µg

Anti-DDIT3 / CHOP Antibody IHC-plus™ - LS-B3652 from LifeSpan Bioscience

Prices $395.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Anti-DDIT3 / GADD153 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3652 concentration 5 ug/ml.

Anti-DDIT3 / CHOP Antibody - LS-C330841 from LifeSpan Bioscience

Prices $255.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Immunohistochemistry of paraffin-embedded rat kidney using DDIT3 antibody at dilution of 1:100 (200x lens).

Anti-DDIT3 / CHOP Antibody (clone 9C8) - LS-C35922 from LifeSpan Bioscience

Prices $665.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 9C8

Anti-DDIT3 / CHOP Antibody (N-Terminus) - LS-C79280 from LifeSpan Bioscience

Host Mouse
Clonality Monoclonal

Anti-DDIT3 / CHOP Antibody (clone 9C8, Biotin) - LS-C180575 from LifeSpan Bioscience

Prices $365.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 9C8

Anti-DDIT3 / CHOP Antibody (clone 9C8, DY488) - LS-C180577 from LifeSpan Bioscience

Prices $365.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 9C8
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