Anti-DDR2 antibody

• Supplier: Abcam
• Catalog: ab76967
• Prices: $378.00
• Sizes: 100 µg
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Applications: WB IHC-P
• Species Reactivities: Mouse, Rat, Bovine, Dog, Human, Chimpanzee, Monkey
• Antigen: Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human DDR2
• Description: Rabbit Polyclonal
• Gene: DDR2
• Conjugate: Unconjugated

Product images

All lanes : Anti-DDR2 antibody (ab76967) at 1 µg/mlLane 1 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate Lane 2 : Liver (Rat) Tissue LysateLane 3 : Pancreas (Mouse) Tissue LysateLysates/proteins at 10 µg per lane.SecondaryGoat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.

IHC image of DDR2 staining in human heart muscle formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab76967, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Product References

The proteome of mouse cerebral arteries. - The proteome of mouse cerebral arteries.

Badhwar A, Stanimirovic DB, Hamel E, Haqqani AS. J Cereb Blood Flow Metab. 2014 Jun;34(6):1033-46.

Discoidin domain receptor 2 regulates neutrophil chemotaxis in 3D collagen - Discoidin domain receptor 2 regulates neutrophil chemotaxis in 3D collagen

Afonso PV, McCann CP, Kapnick SM, Parent CA. Blood. 2013 Feb 28;121(9):1644-50.