ab135730 stained U-87 MG cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab135730 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Anti-Deltex antibody (ab135730) at 1/100 dilution + HeLa cell line lysate at 35 µg
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human lymph tissue labelling Deltex with ab135730 at 1/50 dilution, followed by peroxidase-conjugated secondary antibody and DAB staining.
Flow cytometric analysis of Jurkat cells labelling Deltex with ab135730 at 1/10 dilution (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.