DTX1

Gene Symbol DTX1
Entrez Gene 1840
Alt Symbol hDx-1
Species Human
Gene Type protein-coding
Description deltex 1, E3 ubiquitin ligase
Other Description E3 ubiquitin-protein ligase DTX1|deltex homolog 1|deltex1|hDTX1|protein deltex-1
Swissprots O60630 Q86Y01 Q9BS04
Accessions EAW98028 EAW98029 Q86Y01 AF053700 AAC06246 BC005816 AAH05816 BC008321 BC048216 AAH48216 XM_011538009 XP_011536311 NM_004416 NP_004407
Function Functions as a ubiquitin ligase protein in vivo, mediating ubiquitination and promoting degradation of MEKK1, suggesting that it may regulate the Notch pathway via some ubiquitin ligase activity (By similarity). Regulator of Notch signaling, a signaling pathway involved in cell-cell communications that regulates a broad spectrum of cell-fate determinations. Mainly acts as a positive regulator of Notch, but it also acts as a negative regulator, depending on the developmental and cell context. Mediates the antineural activity of Notch, possibly by inhibiting the transcriptional activation mediated by MATCH1. Involved in neurogenesis, lymphogenesis and myogenesis, and may also be involved in MZB (Marginal zone B) cell differentiation. Promotes B-cell development at the expense of T- cell development, suggesting that it can antagonize NOTCH1. {ECO:0000250, ECO:0000269|PubMed:11564735, ECO:0000269|PubMed:11869684, ECO:0000269|PubMed:9590294}.
Subcellular Location Cytoplasm. Nucleus. Note=Predominantly cytoplasmic. Associates with endocytic vesicles. Partially nuclear.
Tissue Specificity Widely expressed. Strongly expressed in blood vessel. Also expressed in embryonic nervous system, pancreas, lung, adrenal gland, digestive tube and muscles. Expressed in MZB cells and developing B- and T-cells. {ECO:0000269|PubMed:12753744, ECO:0000269|PubMed:9590294}.
Top Pathways Notch signaling pathway

Deltex-1 (D-20) - sc-46089 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
Deltex-1 (D-20): sc-46089. Western blot analysis of Deltex-1 expression in SJRH30 (A), K-562 (B) and Hs 294T (C) whole cell lysates.

Deltex-1 (H-80) - sc-66858 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
Deltex-1 (H-80): sc-66858. Immunoperoxidase staining of formalin fixed, paraffin-embedded human hippocampus tissue showing cytoplasmic staining of neuronal cells and Glial cells.

Anti-Deltex antibody - ab135730 from Abcam

Prices $370.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
ab135730 stained U-87 MG cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab135730 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-Deltex antibody - ab198877 from Abcam

Prices $376.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Immunohistochemical analysis of paraffin-embedded Human cervical cancer tissue labeling Deltex with ab198877 at 1/30 dilution.

Anti-Deltex antibody - ab198185 from Abcam

Prices $370.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Immunohistochemical analysis of formalin fixed paraffin embedded human brain tissue labeling Deltex with ab198185 at 1/20

Anti-Deltex antibody - ab96439 from Abcam

Prices $370.00
Sizes 50 µl
Host Rabbit
Clonality Polyclonal
Anti-Deltex antibody (ab96439) at 1/1000 dilution + HeLa cell lysate at 30 µg

Anti-Deltex antibody - ab157182 from Abcam

Prices $370.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
All lanes : Anti-Deltex antibody (ab157182) at 1/1000 dilutionLane 1 : Mouse brain total cell extractLane 2 : Mouse spleen total cell extractLane 3 : Mouse liver total cell extract

Anti-DTX1 / Deltex Antibody (aa45-57) - LS-C108917 from LifeSpan Bioscience

Prices $335.00
Sizes 100 µg
Host Goat
Clonality Polyclonal

Anti-DTX1 / Deltex Antibody (aa128-144) - LS-C149018 from LifeSpan Bioscience

Host Rabbit
Clonality Polyclonal

Anti-DTX1 / Deltex Antibody (aa583-599) - LS-C149033 from LifeSpan Bioscience

Host Rabbit
Clonality Polyclonal

Anti-DTX1 / Deltex Antibody (Internal, AP) - LS-C276905 from LifeSpan Bioscience

Prices $515.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-DTX1 / Deltex Antibody (Internal, Biotin) - LS-C276908 from LifeSpan Bioscience

Prices $515.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-DTX1 / Deltex Antibody (Internal, Azide-free, HRP) - LS-C276911 from LifeSpan Bioscience

Prices $515.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-DTX1 / Deltex Antibody (Internal, PE) - LS-C276912 from LifeSpan Bioscience

Prices $515.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-DTX1 / Deltex Antibody (Internal, FITC) - LS-C276909 from LifeSpan Bioscience

Prices $515.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-DTX1 / Deltex Antibody (Internal, APC) - LS-C276907 from LifeSpan Bioscience

Prices $515.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-DTX1 / Deltex Antibody (aa382-410) - LS-C327224 from LifeSpan Bioscience

Prices $345.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-DTX1 / Deltex Antibody (aa35-84) - LS-C110276 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
DTX1 / Deltex antibody LS-C110276 Western blot of THP-1 cell lysate.

Anti-DTX1 / Deltex Antibody (aa382-410) - LS-C167556 from LifeSpan Bioscience

Prices $295.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
Formalin-fixed and paraffin-embedded human lymph tissue reacted with DTX1 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Anti-DTX1 / Deltex Antibody (aa343-617) - LS-C186262 from LifeSpan Bioscience

Prices $335.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
IHC of paraffin-embedded BT474 xenograft using Deltex1 antibody at 1:500 dilution.

DTX1/DTX4 Antibody (730612) [Unconjugated] - MAB7157 from Novus Biologicals

Prices $99.00, $249.00
Sizes 25 µg, 100 µg
Host Mouse
Clonality Monoclonal
Clone 730612
Western blot shows lysates of K562 human chronic myelogenous leukemia cell line and SW13 human adrenal cortex adenocarcinoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human DTX1/DTX4 Monoclonal Antibody (Catalog # MAB7157) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for DTX1/DTX4 at approximately 67 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

DTX1 Antibody - NBP2-13940 from Novus Biologicals

Prices $419.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Immunohistochemistry-Paraffin: DTX1 Antibody [NBP2-13940] Staining of human hippocampus shows strong cytoplasmic and nuclear positivity in glial cells.

DTX1 Antibody - NBP1-46126 from Novus Biologicals

Prices $229.00
Sizes 100 µg
Host Goat
Clonality Polyclonal

Deltex 1 Polyclonal Antibody - bs-0399R from Bioss

Prices $269.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Formalin-fixed and paraffin-embedded rat brain labeled with Anti-Deltex 1 Polyclonal Antibody, Unconjugated(bs-0399R) 1:200, overnight at 4°C, The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated(bs-0295G-Cy3)used at 1:200 dilution for 40 minutes at 37°C.DAPI was used to stain the cell nuclei

Deltex 1 Polyclonal Antibody, ALEXA FLUOR® 350 Conjugated - bs-0399R-A350 from Bioss

Prices $369.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Search more