Anti-Desmoplakin I+II antibody
| Name | Anti-Desmoplakin I+II antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab71690 |
| Prices | $387.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | WB ICC/IF ICC/IF |
| Species Reactivities | Human, Xenopus |
| Antigen | Synthetic peptide derived from within residues 1 - 100 of Human Desmoplakin I+II |
| Description | Rabbit Polyclonal |
| Gene | DSP |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Desmoplakin I+II antibody (ab71690) at 1 µg/ml + Caco 2 (Human colonic carcinoma cell line) Whole Cell Lysate at 10 µgSecondaryGoat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
ICC/IF image of ab71690 stained HepG2 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal Goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab71690, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 Goat anti-Rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% PFA fixed (10 min) HeLa, Hek293, MCF-7 cells at 5µg/ml.
Product References
Synergy analysis reveals association between insulin signaling and desmoplakin - Synergy analysis reveals association between insulin signaling and desmoplakin
Wang X, Nath A, Yang X, Portis A, Walton SP, Chan C. PLoS One. 2011;6(11):e28138.
Protruding membrane nanotubes: attachment of tubular protrusions to adjacent - Protruding membrane nanotubes: attachment of tubular protrusions to adjacent
Lokar M, Iglic A, Veranic P. Protoplasma. 2010 Oct;246(1-4):81-7.