Anti-DHCR7 antibody
| Name | Anti-DHCR7 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab103296 |
| Prices | $382.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | ICC/IF ICC/IF WB |
| Species Reactivities | Mouse, Rat, Human |
| Antigen | Fragment protein corresponding to amino acids 1-259 of Human DHCR7 (NP_001351 |
| Description | Rabbit Polyclonal |
| Gene | DHCR7 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ICC/IF image of ab103296 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab103296, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Anti-DHCR7 antibody (ab103296) at 1/500 dilution + Rat brain tissue lysate at 50 µg
Anti-DHCR7 antibody (ab103296) at 1/500 dilution + Mouse testis tissue lysate at 50 µg
Anti-DHCR7 antibody (ab103296) at 1/500 dilution + HepG2 cell lysate at 50 µg
Product References
The endoplasmic reticulum coat protein II transport machinery coordinates - The endoplasmic reticulum coat protein II transport machinery coordinates
Fryer LG, Jones B, Duncan EJ, Hutchison CE, Ozkan T, Williams PA, Alder O, Nieuwdorp M, Townley AK, Mensenkamp AR, Stephens DJ, Dallinga-Thie GM, Shoulders CC. J Biol Chem. 2014 Feb 14;289(7):4244-61.
Prominent steatosis with hypermetabolism of the cell line permissive for years of - Prominent steatosis with hypermetabolism of the cell line permissive for years of
Sugiyama K, Ebinuma H, Nakamoto N, Sakasegawa N, Murakami Y, Chu PS, Usui S, Ishibashi Y, Wakayama Y, Taniki N, Murata H, Saito Y, Fukasawa M, Saito K, Yamagishi Y, Wakita T, Takaku H, Hibi T, Saito H, Kanai T. PLoS One. 2014 Apr 9;9(4):e94460.
The role of oxidative stress in nervous system aging. - The role of oxidative stress in nervous system aging.
Sims-Robinson C, Hur J, Hayes JM, Dauch JR, Keller PJ, Brooks SV, Feldman EL. PLoS One. 2013 Jul 2;8(7):e68011.