Anti-DHX58 antibody
| Name | Anti-DHX58 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab67270 |
| Prices | $378.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | WB ICC/IF ICC/IF |
| Species Reactivities | Mouse, Rat, Human |
| Antigen | A 12 amino acid synthetic peptide from near the carboxy terminus of human DHX58 |
| Description | Rabbit Polyclonal |
| Gene | DHX58 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ICC/IF image of ab67270 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab67270, 10µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Lane 1 : Anti-DHX58 antibody (ab67270) at 1 µg/mlLane 2 : Anti-DHX58 antibody (ab67270) at 2 µg/mlLane 1 : Rat kidney tissue lysateLane 2 : Rat kidney tissue lysateLysates/proteins at 15 µg per lane.SecondaryAnti rabbit secondary antibody
Product References
LGP2 plays a critical role in sensitizing mda-5 to activation by double-stranded - LGP2 plays a critical role in sensitizing mda-5 to activation by double-stranded
Childs KS, Randall RE, Goodbourn S. PLoS One. 2013 May 9;8(5):e64202.
Paramyxovirus V proteins interact with the RNA Helicase LGP2 to inhibit - Paramyxovirus V proteins interact with the RNA Helicase LGP2 to inhibit
Childs K, Randall R, Goodbourn S. J Virol. 2012 Apr;86(7):3411-21.