DHX58

Gene Symbol DHX58
Entrez Gene 79132
Alt Symbol D11LGP2, D11lgp2e, LGP2, RLR-3
Species Human
Gene Type protein-coding
Description DEXH (Asp-Glu-X-His) box polypeptide 58
Other Description RIG-I-like receptor 3|RIG-I-like receptor LGP2|RLR|RNA helicase LGP2|ortholog of mouse D11lgp2|probable ATP-dependent RNA helicase DHX58|probable ATP-dependent helicase LGP2|protein D11Lgp2 homolog
Swissprots Q96C10 Q9HAM6
Accessions EAW60796 EAW60797 EAW60798 EAW60799 EAW60800 Q96C10 AK021416 BAB13818 AK097669 BAG53507 AK225549 AK296840 BAH12441 AK300900 BAG62538 BC014949 AAH14949 BM723672 JF432115 ADZ15332 XM_011525224 XP_011523526 XR_934548 NM_024119 NP_077024
Function Acts as a regulator of DDX58/RIG-I and IFIH1/MDA5 mediated antiviral signaling. Cannot initiate antiviral signaling as it lacks the CARD domain required for activating MAVS/IPS1- dependent signaling events. Can have both negative and positive regulatory functions related to DDX58/RIG-I and IFIH1/MDA5 signaling and this role in regulating signaling may be complex and could probably depend on characteristics of the infecting virus or target cells, or both. Its inhibitory action on DDX58/RIG-I signaling may involve the following mechanisms: competition with DDX58/RIG-I for binding to the viral RNA, binding to DDX58/RIG-I and inhibiting its dimerization and interaction with MAVS/IPS1, competing with IKBKE in its binding to MAVS/IPS1 thereby inhibiting activation of interferon regulatory factor 3 (IRF3). Its positive regulatory role may involve unwinding or stripping nucleoproteins of viral RNA thereby facilitating their recognition by DDX58/RIG-I and IFIH1/MDA5. Involved in the innate immu
Subcellular Location Cytoplasm {ECO:0000269|PubMed:19380577}.
Top Pathways RIG-I-like receptor signaling pathway

LGP2 (D3I3L) Rabbit mAb - 12869 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D3I3L
Western blot analysis of extracts from various cell lines, untreated (-) or treated with Human Interferon-α1 (hIFNα) #8927 (10 ng/ml, overnight; +), using LGP2 (D3I3L) Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower).

LGP2 (H-159) - sc-134667 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
LGP2 (H-159): sc-134667. Western blot analysis of LGP2 expression in mouse liver (A) and mouse thymus (B) tissue extracts.

LGP2 (C-9) - sc-373827 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
LGP2 (C-9): sc-373827. Western blot analysis of LGP2 expression in non-transfected (A) and human LGP2 transfected (B) whole cell lysates.

LGP2 (M-159) - sc-134668 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
LGP2 (M-159): sc-134668. Western blot analysis of LGP2 expression in mouse liver (A) and mouse thymus (B) tissue extracts.

LGP2 (N-14) - sc-164861 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
LGP2 (N-14): sc-164861. Western blot analysis of LGP2 expression in HeLa (A), MCF7 (B), HEK293 (C) and A549 (D) whole cell lysates.

LGP2 (E-1) - sc-373826 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
LGP2 (E-1): sc-373826. Western blot analysis of LGP2 expression in non-transfected (A) and human LGP2 transfected (B) whole cell lysates.

Anti-DHX58 antibody - ab67270 from Abcam

Prices $378.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
ICC/IF image of ab67270 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab67270, 10µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-DHX58 antibody - ab100882 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Anti-DHX58 antibody (ab100882) at 1 µg/ml + MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate at 10 µgSecondaryGoat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.

Anti-DHX58 antibody - ab115674 from Abcam

Prices $378.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Lane 1 : Anti-DHX58 antibody (ab115674) at 1 µg/mlLane 2 : Anti-DHX58 antibody (ab115674) at 2 µg/mlLane 1 : Rat kidney tissue lysateLane 2 : Rat kidney tissue lysate

Anti-DHX58 antibody - ab67272 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Lane 1 : Anti-DHX58 antibody (ab67272) at 1 µg/mlLane 2 : Anti-DHX58 antibody (ab67272) at 2 µg/mlLane 1 : Rat kidney tissue lysateLane 2 : Rat kidney tissue lysateLysates/proteins at 15 µg per lane.Secondaryanti rabbit secondary antibody

Anti-DHX58 antibody - ab168127 from Abcam

Prices $376.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal
All lanes : Anti-DHX58 antibody (ab168127) at 1 µg/mlLane 1 : DHX58 transfected 293T cell lysateLane 2 : Non-transfected 293T cell lysateLysates/proteins at 15 µl per lane.SecondaryGoat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

Anti-DHX58 antibody - ab82151 from Abcam

Prices $376.00
Sizes 100 µg
Host Goat
Clonality Polyclonal
Anti-DHX58 antibody (ab82151) at 0.3 µg/ml + Human liver lysate in RIPA buffer at 35 µg

Anti-DHX58 antibody - ab112546 from Abcam

Prices $370.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal

Anti-DHX58 / LGP2 Antibody (C-Terminus) - LS-C53209 from LifeSpan Bioscience

Prices $325.00, $890.00
Sizes 50 µg, 500 µg
Host Rabbit
Clonality Polyclonal
Western blot of LGP2 in rat kidney tissue lysate with LGP2 antibody at (A) 1 and (B) 2 ug/ml.

Anti-DHX58 / LGP2 Antibody (N-Terminus) - LS-C53210 from LifeSpan Bioscience

Prices $325.00, $890.00
Sizes 50 µg, 500 µg
Host Rabbit
Clonality Polyclonal
Western blot of LGP2 in MDA-MB-361 cell lysate with LGP2 antibody at (A) 0.5 and (B) 1 ug/ml.

Anti-DHX58 / LGP2 Antibody (aa663-674) - LS-C87220 from LifeSpan Bioscience

Prices $395.00
Sizes 100 µg
Host Goat
Clonality Polyclonal
Antibody (0.3 ug/ml) staining of Human Liver lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence

Anti-DHX58 / LGP2 Antibody (aa41-69, AP) - LS-C234586 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-DHX58 / LGP2 Antibody (aa647-659) - LS-C194005 from LifeSpan Bioscience

Prices $905.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-DHX58 / LGP2 Antibody (aa35-84) - LS-C110169 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
DHX58 antibody LS-C110169 Western blot of Fetal Spleen lysate.

Anti-DHX58 / LGP2 Antibody (aa41-69, Biotin) - LS-C234587 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-DHX58 / LGP2 Antibody (aa41-69, FITC) - LS-C234588 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-DHX58 / LGP2 Antibody (aa41-69, PE) - LS-C234589 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-DHX58 / LGP2 Antibody (aa41-69, APC) - LS-C246846 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-DHX58 / LGP2 Antibody (aa41-69, Azide-free, HRP) - LS-C249831 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-DHX58 / LGP2 Antibody - LS-C310130 from LifeSpan Bioscience

Prices $475.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
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