Anti-DIO2 antibody (ab135711) at 1/100 dilution + MCF7 cell line lysate at 35 µg
Immunohistochemical analysis of formalin-fixed, paraffin-embedded mouse brain tissue labelling DIO2 with ab135711 at 1/50 dilution, followed by peroxidase-conjugated secondary antibody and DAB staining.
Flow Cytometric analysis of MCF7 cells labelling DIO2 with ab135711 at 1/10 dilution (bottom histogram), compared to negative control cells (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.