All lanes : Anti-Dis3 antibody (ab64289) at 1/100 dilutionLane 1 : S. pombe cell lysate transformed with the vectorLane 2 : S. pombe cell lysate transformed with a plasmid carrying truncated gene (172)Lane 3 : S. pombe cell lysate transformed with a plasmid carrying truncated gene (A)Lane 4 : S. pombe cell lysate transformed with a plasmid carrying truncated gene (B)Lane 5 : S. pombe cell lysate transformed with a plasmid carrying truncated gene (C)Lane 6 : S. pombe cell lysate transformed with a plasmid carrying truncated gene (E)