Anti-DKK1 antibody (ab88334) at 1 µg/ml + HeLa cell lysate at 25 µgSecondaryGoat anti-Mouse IgG at 1/5000 dilution
Immunoperoxidase staining of DKK1 using ab88334 at 3 µg/ml on formalin-fixed, paraffin-embedded Human placenta tissue.
ICC/IF image of ab88334 stained MCF-7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab88334, 10µg/ml) overnight at +4°C. The secondary antibody (green) was ab96879 Dylight 488 goat anti-mouse IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.