Anti-Dkk3 antibody [EPR15611]

• Supplier: Abcam
• Catalog: ab186409
• Prices: $384.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: EPR15611
• Applications: WB ICC/IF ICC/IF IP
• Species Reactivities: Mouse, Rat, Human
• Antigen: Recombinant fragment within Human Dkk3 aa 250 to the C-terminus
• Description: Rabbit Monoclonal
• Gene: DKK3
• Conjugate: Unconjugated

Product images

Anti-Dkk3 antibody [EPR15611] (ab186409) at 1/10000 dilution + Human fetal brain lysate at 20 µgSecondaryGoat anti-rabbit IgG, (H+L), peroxidase conjugate at 1/1000 dilution

All lanes : Anti-Dkk3 antibody [EPR15611] (ab186409) at 1/5000 dilutionLane 1 : Human fetal kidney lysateLane 2 : Human fetal heart lysateLysates/proteins at 10 µg per lane.SecondaryAnti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Immunofluorescent analysis of 4% paraformaldehyde-fixed HepG2 cells labeling Dkk3 with ab186409 at 1/100 dilution, followed by Goat anti rabbit IgG (Alexa Fluor® 488) secondary antibody at 1/200 dilution. Counter stained with Dapi (blue).

Western blot analysis of Dkk3 in Human fetal brain lysate immunoprecipitated with ab186409 at 1/50 dilution (Lane 1). Lane 2: PBS instead of Human fetal brain lysate.Secondary antibody: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.