All lanes : Anti-DLL4 antibody [EPR3540(N)] (ab185232) at 1/1000 dilutionLane 1 : Human fetal kidney lysateLane 2 : Hela cell lysateLane 3 : HepG2 cell lysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
All lanes : Anti-DLL4 antibody [EPR3540(N)] (ab185232) at 1/1000 dilutionLane 1 : Human skeletal muscle cell lysateLane 2 : Human fetal lung cell lysateLysates/proteins at 10 µg per lane.SecondaryAnti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution