Anti-DMAP1 antibody [EPR18179]

• Supplier: Abcam
• Catalog: ab188407
• Prices: $347.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: EPR18179
• Applications: ICC/IF ICC/IF WB
• Species Reactivities: Mouse, Rat, Human
• Antigen: Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human DMAP1 aa 400 to the C-terminus
• Description: Rabbit Monoclonal
• Gene: DMAP1
• Conjugate: Unconjugated

Product images

All lanes : Anti-DMAP1 antibody [EPR18179] (ab188407) at 1/1000 dilutionLane 1 : HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysateLane 2 : Jurkat (Human T cell leukemia cells from peripheral blood) whole cell lysateLane 3 : 293 (Human epithelial cells from embryonic kidney) whole cell lysateLane 4 : MCF7 (Human breast adenocarcinoma cell line) whole cell lysateLane 5 : Ramos (Human Burkitt's lymphoma cell line) whole cell lysateLane 6 : Neuro-2a (Mouse neuroblastoma cells) whole cell lysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

All lanes : Anti-DMAP1 antibody [EPR18179] (ab188407) at 1/1000 dilutionLane 1 : Raw264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) whole cell lysateLane 2 : PC12 (Rat adrenal gland pheochromocytoma) whole cell lysateLane 3 : NIH 3T3 (Mouse embyro fibroblast cells) whole cell lysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

All lanes : Anti-DMAP1 antibody [EPR18179] (ab188407) at 1/2000 dilutionLane 1 : HeLa (Human epithelial cells from cervix adenocarcinoma) cytosolic fraction lysateLane 2 : HeLa (Human epithelial cells from cervix adenocarcinoma) nuclear fraction lysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cells from cervix adenocarcinoma) cells labeling DMAP1 with ab188407 at 1/2000 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).Confocal image showing nuclear staining on HeLa cell line.The nuclear counter stain is DAPI (blue).Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution (red).The negative controls are as follows:1. ab188407 at 1/2000 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution.2. ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/1000 dilution.

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized F9 (Mouse embryonic carcinoma cell line) cells labeling DMAP1 with ab188407 at 1/2000 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).Confocal image showing nuclear staining on F9 cell line.The nuclear counter stain is DAPI (blue).Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution (red).The negative controls are as follows:1. ab188407 at 1/2000 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution.2. ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/1000 dilution.