Anti-DMAP1 antibody - ChIP Grade

• Supplier: Abcam
• Catalog: ab2848
• Prices: $380.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Applications: ChIP IHC-P WB ICC/IF ICC/IF
• Species Reactivities: Mouse, Human, Xenopus
• Antigen: Synthetic peptide corresponding to Mouse DMAP1 aa 435-450
• Description: Rabbit Polyclonal
• Gene: DMAP1
• Conjugate: Unconjugated

Product images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of DMAP1 showing staining in the cytoplasm and nucleus of paraffin-treated human esophageal carcinoma tissue (right) compared with a negative control in the absence of primary antibody (left). Antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then incubated with ab2848 diluted by 3% BSA-PBS at a dilution of 1:100 overnight at 4°C in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of DMAP1 showing staining in the cytoplasm and nucleus of paraffin-treated mouse brain tissue (right) compared with a negative control in the absence of primary antibody (left). Antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then incubated with ab2848 diluted by 3% BSA-PBS at a dilution of 1:100 overnight at 4°C in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of DMAP1 showing staining in the cytoplasm and nucleus of paraffin-treated mouse uterus tissue (right) compared with a negative control in the absence of primary antibody (left). Antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then incubated with ab2848 diluted by 3% BSA-PBS at a dilution of 1:100 overnight at 4°C in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.

All lanes : Anti-DMAP1 antibody - ChIP Grade (ab2848) at 1/1000 dilutionLane 1 : HL-60 cell lysateLane 2 : HeLa cell lysateLane 3 : Mouse brain cell lysateLysates/proteins at 25 µg per lane.

ab2848 staining DMAP1 in asynchronous HeLa cells in conjunction with a secondary goat anti-rabbit antibody conjugated to Cy3 ® (green). Cells were counterstained with DAPI (red) to show the nucleus. Please refer to abreview for further details.See Abreview

Anti-DMAP1 antibody - ChIP Grade (ab2848) at 1/500 dilution + whole cell lysate prepared from Xenopus laevis egg at 10 µgSecondaryHRP-Protein A at 1/1000 dilutiondeveloped using the ECL technique

Product References

Knockout of mouse Cyp3a gene enhances synthesis of cholesterol and bile acid in - Knockout of mouse Cyp3a gene enhances synthesis of cholesterol and bile acid in

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Distinct roles of DMAP1 in mouse development. - Distinct roles of DMAP1 in mouse development.

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DNA methyltransferase 1-associated protein (DMAP1) is a co-repressor that - DNA methyltransferase 1-associated protein (DMAP1) is a co-repressor that

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Dmap1 plays an essential role in the maintenance of genome integrity through the - Dmap1 plays an essential role in the maintenance of genome integrity through the

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Bmi1 cooperates with Dnmt1-associated protein 1 in gene silencing. - Bmi1 cooperates with Dnmt1-associated protein 1 in gene silencing.

Negishi M, Saraya A, Miyagi S, Nagao K, Inagaki Y, Nishikawa M, Tajima S, Koseki H, Tsuda H, Takasaki Y, Nakauchi H, Iwama A. Biochem Biophys Res Commun. 2007 Feb 23;353(4):992-8. Epub 2006 Dec 29.