Western blot analysis of extracts from various cell lines using Atg4B (D1G2R) Rabbit mAb.
Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with a construct expressing full-length mouse Atg4B (mAtg4B; +), using Atg4B (D1G2R) Rabbit mAb.
Western blot analysis of extracts from RD cells, transfected with 100 nM SignalSilence ® Control siRNA (Unconjugated) #6568 (-) or SignalSilence ® Atg4B siRNA I #6336 (+), using Atg4B (D1G2R) Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower). The Atg4B (D1G2R) Rabbit mAb confirms silencing of Atg4B expression, while the β-Actin (D6A8) Rabbit mAb is used as a loading control.
Immunoprecipitation of Atg4B from RD cell extracts using Rabbit (DA1E) mAb IgG XP ® Isotype control #3900 (lane 2) or Atg4B (D1G2R) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using Atg4B (D1G2R) Rabbit mAb.