ATG4B

Gene Symbol	ATG4B
Entrez Gene	23192
Alt Symbol	APG4B, AUTL1
Species	Human
Gene Type	protein-coding
Description	autophagy related 4B, cysteine peptidase
Other Description	APG4 autophagy 4 homolog B\|ATG4 autophagy related 4 homolog B\|AUT-like 1 cysteine endopeptidase\|autophagin-1\|autophagy-related cysteine endopeptidase 1\|autophagy-related protein 4 homolog B\|cysteine protease ATG4B\|hAPG4B
Swissprots	Q6ZUV8 Q53NU4 Q96SZ1 Q96K96 Q9Y2F2 Q96K07 Q8WYM9 Q9Y4P1 B7WNK2
Accessions	AAY14919 EAW71278 EAW71279 EAW71280 EAW71281 Q9Y4P1 AB023160 BAA76787 AB066215 BAB83890 AJ504652 CAD43219 AK027332 BAB55042 AK027462 BAB55127 AK027763 BAB55353 AK123425 BAG53904 AK125277 BAC86110 AK296818 BAG59391 AK298109 BAG60394 AK302963 BAG64112 AK316167 BAH14538 AL050288 AL080168 CAB45756 AL133638 AM393751 CAL38626 BC000719 AAH00719 BX648007 CAH56198 DQ891869 ABM82795 XM_005246992 XP_005247049 XM_005246993 XP_005247050 XM_005246995 XP_005247052 XM_005246996 XP_005247053 XM_011510836 XP_011509138 XM_011510837 XP_011509139 XM_011510838 XP_011509140 XM_011510839 XP_011509141 NM_013325 NP_037457 NM_178326 NP_847896
Function	Cysteine protease required for the cytoplasm to vacuole transport (Cvt) and autophagy. Cleaves the C-terminal amino acid of ATG8 family proteins MAP1LC3, GABARAPL1, GABARAPL2 and GABARAP, to reveal a C-terminal glycine. Exposure of the glycine at the C- terminus is essential for ATG8 proteins conjugation to phosphatidylethanolamine (PE) and insertion to membranes, which is necessary for autophagy. Has also an activity of delipidating enzyme for the PE-conjugated forms. {ECO:0000269\|PubMed:15169837, ECO:0000269\|PubMed:15187094, ECO:0000269\|PubMed:17347651, ECO:0000269\|PubMed:19322194, ECO:0000269\|PubMed:21177865, ECO:0000269\|PubMed:22302004}.
Subcellular Location	Cytoplasm {ECO:0000250}.
Tissue Specificity	Mainly expressed in the skeletal muscle, followed by brain, heart, liver and pancreas. {ECO:0000269\|PubMed:12446702, ECO:0000269\|PubMed:15169837}.
Top Pathways	Regulation of autophagy

Atg4B (D1G2R) Rabbit mAb - 13507 from Cell Signaling Technology

Prices	$246.00
Sizes	100 µl (10 western blots)
Host	Rabbit
Clonality	Monoclonal
Clone	D1G2R

Western blot analysis of extracts from various cell lines using Atg4B (D1G2R) Rabbit mAb.

Atg4B Antibody - 5299 from Cell Signaling Technology

Prices	$246.00
Sizes	100 µl (10 western blots)
Host	Rabbit
Clonality	Polyclonal

Western blot analysis of extracts from various cell lines using Atg4B Antibody.

Atg4b (I-13) - sc-83371 from Santa Cruz Biotechnology

Prices	$279.00
Sizes	200 µg/ml
Host	Goat
Clonality	Polyclonal

Atg4b (I-13): sc-83371. Western blot analysis of Atg4b expression in Jurkat (A), HeLa (B), HEK293 (C) and MCF7 (D) whole cell lysates and human brain (E) and mouse brain (F) tissue extracts.

Atg4b (H-57) - sc-367838 from Santa Cruz Biotechnology

Prices	$279.00
Sizes	200 µg/ml
Host	Rabbit
Clonality	Polyclonal

Atg4b (H-57): sc-367838. Western blot analysis of Atg4b expression in mouse brain tissue extract.

Atg4b (K-25) - sc-130968 from Santa Cruz Biotechnology

Prices	$279.00
Sizes	50 µg/0.5 ml
Host	Rabbit
Clonality	Polyclonal

Atg4b (K-25): sc-130968. Western blot analysis of Atg4b expression in 293T whole cell lysate.

Anti-ATG4B antibody - ab69937 from Abcam

Prices	$380.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

Immunohistochemical analysis of paraffin embedded human skeletal muscle tissue labelled with ab69937 at 1/50-1/100 dilution. Right panel was also treated with immunising peptide.

Anti-ATG4B antibody - ab70867 from Abcam

Prices	$370.00
Sizes	400 µl
Host	Rabbit
Clonality	Polyclonal

All lanes : Anti-ATG4B antibody (ab70867) at 1/50 dilutionLane 1 : 293 cell lysates (nontransfected)Lane 2 : 293 cell lysates transiently transfected with the ATG4B gene Lysates/proteins at 2 µg per lane.

Anti-ATG4B antibody - ab64737 from Abcam

Host	Sheep
Clonality	Polyclonal

Anti-ATG4B antibody - ab71010 from Abcam

Prices	$359.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

All lanes : Anti-ATG4B antibody (ab71010) at 1/50 dilutionLane 1 : Nontransfected 293 cell lysatesLane 2 : 293 cell lysates transiently transfected with the ATG4B geneLysates/proteins at 2 µg per lane.

Anti-ATG4B antibody - ab72878 from Abcam

Prices	$370.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

All lanes : Anti-ATG4B antibody (ab72878) at 1 µg/mlLane 1 : Heart (Human) Tissue Lysate - adult normal tissue (ab29431)Lane 2 : Liver (Human) Tissue Lysate - adult normal tissue (ab29889)Lysates/proteins at 10 µg per lane.SecondaryGoat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.

Anti-ATG4B antibody - ab101983 from Abcam

Prices	$370.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

Detection of ATG4B by Western Blot of Immunprecipitate. An antibody to ATG4B, at 1 µg/ml, staining ATG4B in HeLa whole cell lysate immunoprecipitated using ab101983 at 3 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Detection: Chemiluminescence with an exposure time of 30 seconds.

Anti-ATG4B antibody - ab72201 from Abcam

Prices	$370.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

ab72201 at 10µg/ml staining ATG4B in human heart by Immunohistochemistry, Paraffin-embedded tissue.

Anti-ATG4B antibody [EPR6436(2)] - ab154843 from Abcam

Prices	$357.00
Sizes	100 µl
Host	Rabbit
Clonality	Monoclonal
Clone	EPR6436(2)

All lanes : Anti-ATG4B antibody [EPR6436(2)] (ab154843) at 1/1000 dilutionLane 1 : Ramos cell lysateLane 2 : 293T cell lysateLane 3 : Jurkat cell lysateLane 4 : HeLa cell lysateLysates/proteins at 10 µg per lane.

Anti-APG4B / ATG4B Antibody (N-Terminus) - LS-C69118 from LifeSpan Bioscience

Prices	$415.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

Anti-APG4B / ATG4B Antibody (C-Terminus) - LS-C31597 from LifeSpan Bioscience

Prices	$325.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

Anti-APG4B / ATG4B Antibody - LS-C47395 from LifeSpan Bioscience

Prices	$335.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

Western blot of APG4B in Jurkat cell lysate. Lane 1 shows pre-immune sera (1:5000) and lane 2 shows antibody at 3 ug/ml.

Anti-APG4B / ATG4B Antibody (C-Terminus) - LS-C29815 from LifeSpan Bioscience

Prices	$365.00
Sizes	50 µg
Host	Rabbit
Clonality	Polyclonal

ATG4B antibody LS-C29815 was used in IHC to stain formalin-fixed, paraffin-embedded human kidney.

Anti-APG4B / ATG4B Antibody (aa239-265, AP) - LS-C251502 from LifeSpan Bioscience

Prices	$545.00
Sizes	200 µl
Host	Rabbit
Clonality	Polyclonal

Anti-APG4B / ATG4B Antibody (aa239-265, APC) - LS-C251503 from LifeSpan Bioscience

Prices	$545.00
Sizes	200 µl
Host	Rabbit
Clonality	Polyclonal

Anti-APG4B / ATG4B Antibody (aa239-265, FITC) - LS-C251505 from LifeSpan Bioscience

Prices	$545.00
Sizes	200 µl
Host	Rabbit
Clonality	Polyclonal

Anti-APG4B / ATG4B Antibody (aa72-121) - LS-C81992 from LifeSpan Bioscience

Prices	$365.00
Sizes	50 µg
Host	Rabbit
Clonality	Polyclonal

Placenta lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%.

Anti-APG4B / ATG4B Antibody (aa239-265, Azide-free, HRP) - LS-C251506 from LifeSpan Bioscience

Prices	$545.00
Sizes	200 µl
Host	Rabbit
Clonality	Polyclonal

Anti-APG4B / ATG4B Antibody (aa239-265) - LS-C159088 from LifeSpan Bioscience

Prices	$295.00
Sizes	400 µl
Host	Rabbit
Clonality	Polyclonal

APG4B Antibody (Center G254)western blot of Uterus tissue lysates (35 ug/lane). The APG4B antibody detected the APG4B protein (arrow).

Anti-APG4B / ATG4B Antibody (aa239-265, PE) - LS-C251507 from LifeSpan Bioscience

Prices	$545.00
Sizes	200 µl
Host	Rabbit
Clonality	Polyclonal

Anti-APG4B / ATG4B Antibody (aa258-284, Biotin) - LS-C251933 from LifeSpan Bioscience

Prices	$545.00
Sizes	200 µl
Host	Rabbit
Clonality	Polyclonal

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