Anti-DNA Ligase III antibody [6G9]
| Name | Anti-DNA Ligase III antibody [6G9] |
|---|---|
| Supplier | Abcam |
| Catalog | ab587 |
| Prices | $400.00 |
| Sizes | 50 µg |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Clone | 6G9 |
| Applications | IHC-P WB ICC/IF ICC/IF FC |
| Species Reactivities | Human |
| Antigen | Recombinant human DNA Ligase III protein encoding amino acids 1-862 purified from baculovirus-infected Sf-9 cells |
| Description | Mouse Monoclonal |
| Gene | LIG3 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ab587 staining cultured human HeLa cells by ICC/IF. Cells were PFA fixed and permeabilized in 0.5% Triton X100 prior to blocking in 5% BSA for 1 hour at 20°C. The primary antibody was diluted 1/200 and incubated with the sample for 1 hour at 20°C. A Cy3® conjugated donkey anti-mouse antibody diluted was used as the secondary.See Abreview
![Anti-DNA Ligase III antibody [6G9] (ab587) at 1 µg/ml + Testis (Human) Tissue Lysate - adult normal tissue (ab30257) at 10 µgSecondaryGoat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_2/8949_DNA-Ligase-III-Primary-antibodies-ab587-3.jpg)
Anti-DNA Ligase III antibody [6G9] (ab587) at 1 µg/ml + Testis (Human) Tissue Lysate - adult normal tissue (ab30257) at 10 µgSecondaryGoat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.
![Overlay histogram showing HeLa cells stained with ab587 (red line). The cells were fixed with 100% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab587, 1µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_2/8950_DNA-Ligase-III-Primary-antibodies-ab587-4.jpg)
Overlay histogram showing HeLa cells stained with ab587 (red line). The cells were fixed with 100% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab587, 1µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.
Product References
An inverse switch in DNA base excision and strand break repair contributes to - An inverse switch in DNA base excision and strand break repair contributes to
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Involvement of DNA ligase III and ribonuclease H1 in mitochondrial DNA - Involvement of DNA ligase III and ribonuclease H1 in mitochondrial DNA
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Transcriptional regulation of the base excision repair pathway by BRCA1. - Transcriptional regulation of the base excision repair pathway by BRCA1.
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Direct interaction between XRCC1 and UNG2 facilitates rapid repair of uracil in - Direct interaction between XRCC1 and UNG2 facilitates rapid repair of uracil in
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Role of tyrosyl-DNA phosphodiesterase (TDP1) in mitochondria. - Role of tyrosyl-DNA phosphodiesterase (TDP1) in mitochondria.
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An 8-oxo-guanine repair pathway coordinated by MUTYH glycosylase and DNA - An 8-oxo-guanine repair pathway coordinated by MUTYH glycosylase and DNA
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Extracts of proliferating and non-proliferating human cells display different - Extracts of proliferating and non-proliferating human cells display different
Akbari M, Pena-Diaz J, Andersen S, Liabakk NB, Otterlei M, Krokan HE. DNA Repair (Amst). 2009 Jul 4;8(7):834-43.
The rate of base excision repair of uracil is controlled by the initiating - The rate of base excision repair of uracil is controlled by the initiating
Visnes T, Akbari M, Hagen L, Slupphaug G, Krokan HE. DNA Repair (Amst). 2008 Nov 1;7(11):1869-81.
Knockdown of DNA ligase IV/XRCC4 by RNA interference inhibits herpes simplex - Knockdown of DNA ligase IV/XRCC4 by RNA interference inhibits herpes simplex
Muylaert I, Elias P. J Biol Chem. 2007 Apr 13;282(15):10865-72. Epub 2007 Feb 12.
Poly(ADP-ribose) polymerase-1 protects excessive DNA strand breaks from - Poly(ADP-ribose) polymerase-1 protects excessive DNA strand breaks from
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