Anti-DNA Ligase IV antibody

• Supplier: Abcam
• Catalog: ab80514
• Prices: $398.00
• Sizes: 100 µg
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Applications: WB ICC/IF ICC/IF IHC-P
• Species Reactivities: Mouse, Human, Orangutan
• Antigen: Synthetic peptide conjugated to KLH derived from within residues 400 - 500 of Human DNA Ligase IV
• Description: Rabbit Polyclonal
• Gene: LIG4
• Conjugate: Unconjugated

Product images

All lanes : Anti-DNA Ligase IV antibody (ab80514) at 1 µg/mlLane 1 : Testis (Human) Tissue Lysate - adult normal tissue (ab30257)Lane 2 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate Lysates/proteins at 10 µg per lane.SecondaryGoat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.

ICC/IF image of ab80514 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal Goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab80514, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 Goat anti-Rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

IHC image of ab80514 staining in Human Normal Cervix formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab80514, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Product References

Dubowitz syndrome is a complex comprised of multiple, genetically distinct and - Dubowitz syndrome is a complex comprised of multiple, genetically distinct and

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SET8 methyltransferase activity during the DNA double-strand break response is - SET8 methyltransferase activity during the DNA double-strand break response is

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APLF promotes the assembly and activity of non-homologous end joining protein - APLF promotes the assembly and activity of non-homologous end joining protein

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Androgen receptor signaling regulates DNA repair in prostate cancers. - Androgen receptor signaling regulates DNA repair in prostate cancers.

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DNA ligase III is critical for mtDNA integrity but not Xrcc1-mediated nuclear DNA - DNA ligase III is critical for mtDNA integrity but not Xrcc1-mediated nuclear DNA

Gao Y, Katyal S, Lee Y, Zhao J, Rehg JE, Russell HR, McKinnon PJ. Nature. 2011 Mar 10;471(7337):240-4.