LIG4

Gene Symbol	LIG4
Entrez Gene	3981
Alt Symbol	LIG4S
Species	Human
Gene Type	protein-coding
Description	ligase IV, DNA, ATP-dependent
Other Description	DNA joinase\|DNA ligase 4\|DNA ligase IV\|DNA repair enzyme\|polydeoxyribonucleotide synthase [ATP] 4\|polynucleotide ligase\|sealase
Swissprots	Q8TEU5 P49917 Q8IY66
Accessions	AAL77435 EAX09094 EAX09095 EAX09096 P49917 AI809944 AK292419 BAF85108 AK314844 BAG37361 BC037491 AAH37491 BX648833 CD102896 DQ892474 ABM83400 DQ895686 ABM86612 X83441 CAA58467 XM_005254056 XP_005254113 XM_005254057 XP_005254114 XM_005254058 XP_005254115 XM_006719951 XP_006720014 XM_006719952 XP_006720015 XM_011521091 XP_011519393 XM_011521092 XP_011519394 NM_001098268 NP_001091738 NM_002312 NP_002303 NM_206937 NP_996820
Function	Efficiently joins single-strand breaks in a double- stranded polydeoxynucleotide in an ATP-dependent reaction. Involved in DNA non-homologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination. The LIG4-XRCC4 complex is responsible for the NHEJ ligation step, and XRCC4 enhances the joining activity of LIG4. Binding of the LIG4-XRCC4 complex to DNA ends is dependent on the assembly of the DNA- dependent protein kinase complex DNA-PK to these DNA ends. {ECO:0000269\|PubMed:10854421, ECO:0000269\|PubMed:9809069}.
Subcellular Location	Nucleus.
Tissue Specificity	Testis, thymus, prostate and heart.
Top Pathways	Non-homologous end-joining

DNA Ligase IV (D5N5N) Rabbit mAb - 14649 from Cell Signaling Technology

Prices	$246.00
Sizes	100 µl (10 western blots)
Host	Rabbit
Clonality	Monoclonal
Clone	D5N5N

Western blot analysis of extracts from various cell lines using DNA Ligase IV (D5N5N) Rabbit mAb.

DNA Ligase IV (C-18) - sc-11750 from Santa Cruz Biotechnology

Prices	$279.00
Sizes	200 µg/ml
Host	Goat
Clonality	Polyclonal

DNA Ligase IV (C-18): sc-11750. Western blot analysis of DNA Ligase IV expression in non-transfected: sc-117752 (A) and human DNA Ligase IV transfected: sc-159741 (B) 293T whole cell lysates.

DNA Ligase IV (D-8) - sc-271299 from Santa Cruz Biotechnology

Prices	$279.00
Sizes	200 µg/ml
Host	Mouse
Clonality	Monoclonal

DNA Ligase IV (D-8): sc-271299. Western blot analysis of DNA Ligase IV expression in HeLa (A) and Ramos (B) whole cell lysates.

DNA Ligase IV (T-20) - sc-11748 from Santa Cruz Biotechnology

Prices	$279.00
Sizes	200 µg/ml
Host	Goat
Clonality	Polyclonal

DNA Ligase IV (H-300) - sc-28232 from Santa Cruz Biotechnology

Prices	$279.00
Sizes	200 µg/ml
Host	Rabbit
Clonality	Polyclonal

DNA Ligase IV (H-300): sc-28232. Western blot analysis of human recombinant DNA Ligase IV fusion protein.

Anti-DNA Ligase IV antibody - ab80514 from Abcam

Prices	$398.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

All lanes : Anti-DNA Ligase IV antibody (ab80514) at 1 µg/mlLane 1 : Testis (Human) Tissue Lysate - adult normal tissue (ab30257)Lane 2 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate Lysates/proteins at 10 µg per lane.SecondaryGoat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.

Anti-DNA Ligase IV antibody - ab65130 from Abcam

Prices	$378.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

All lanes : Anti-DNA Ligase IV antibody (ab65130) at 1/500 dilutionLane 1 : Extracts from Jurkat cellsLane 2 : Extracts from Jurkat cells, with immunising peptide at 5 µgLysates/proteins at 5 µg per lane.

Anti-DNA Ligase IV antibody - ab137380 from Abcam

Prices	$378.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

Anti-DNA Ligase IV antibody (ab137380) at 1/1000 dilution + MOLT4 cell lysate at 30 µg

Anti-DNA Ligase IV antibody - ab169290 from Abcam

Prices	$376.00
Sizes	50 µg
Host	Mouse
Clonality	Polyclonal

All lanes : Anti-DNA Ligase IV antibody (ab169290) at 1 µg/mlLane 1 : DNA Ligase IV transfected 293T lysateLane 2 : Non-transfected 293T lysateLysates/proteins at 15 µl per lane.SecondaryGoat Anti-Mouse IgG (H&L)-HRP at 1/2500 dilutiondeveloped using the ECL technique

Anti-DNA Ligase IV antibody [EPR16531] - ab193353 from Abcam

Prices	$99.00
Sizes	10 µl
Host	Rabbit
Clonality	Monoclonal
Clone	EPR16531

All lanes : Anti-DNA Ligase IV antibody [EPR16531] (ab193353) at 1/1000 dilutionLane 1 : HeLa cell lysateLane 2 : Jurkat cell lysateLane 3 : Ramos cell lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Anti-LIG4 / DNA Ligase IV Antibody (aa275-324) - LS-C121177 from LifeSpan Bioscience

Prices	$635.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

Anti-LIG4 / DNA Ligase IV Antibody (clone 1A4) - LS-C197616 from LifeSpan Bioscience

Prices	$355.00
Sizes	100 µg
Host	Mouse
Clonality	Monoclonal
Clone	1A4

Anti-LIG4 / DNA Ligase IV Antibody (aa616-665) - LS-C291228 from LifeSpan Bioscience

Prices	$315.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

Anti-LIG4 / DNA Ligase IV Antibody (Gly619) - LS-C176883 from LifeSpan Bioscience

Prices	$345.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

Western blot of DNA Ligase IV (G619) pAb in extracts from Jurkat cells.

Anti-LIG4 / DNA Ligase IV Antibody (phospho-Thr650) - LS-C291176 from LifeSpan Bioscience

Prices	$315.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

Anti-LIG4 / DNA Ligase IV Antibody (aa275-324) - LS-C31455 from LifeSpan Bioscience

Prices	$325.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

LIG4 antibody LS-C31455 was used in IHC to stain formalin-fixed, paraffin-embedded human pancreas.

Anti-LIG4 / DNA Ligase IV Antibody (aa591-640) - LS-C119547 from LifeSpan Bioscience

Prices	$315.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

Western blot of extracts from Jurkat cells, using DNL4 Antibody. The lane on the right is treated with the synthesized peptide.

Anti-LIG4 / DNA Ligase IV Antibody (aa239-267) - LS-C203791 from LifeSpan Bioscience

Prices	$295.00
Sizes	400 µl
Host	Rabbit
Clonality	Polyclonal

LIG4 Antibody western blot of human placenta tissue lysates (35 ug/lane). The LIG4 antibody detected the LIG4 protein (arrow).

Anti-LIG4 / DNA Ligase IV Antibody - LS-C192823 from LifeSpan Bioscience

Prices	$385.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

Western blot of extracts of various cell lines, using LIG4 antibody.

Anti-LIG4 / DNA Ligase IV Antibody - LS-C331665 from LifeSpan Bioscience

Prices	$255.00
Sizes	50 µg
Host	Rabbit
Clonality	Polyclonal

Western blot analysis of extracts of various cell lines, using LIG4 antibody.

DNA Ligase IV Antibody - NB110-57379 from Novus Biologicals

Prices	$299.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

Western Blot: DNA Ligase IV Antibody [NB110-57379] - Western blot on Hep G2 cell lysate stained with NB110-57379 at 1.25ug/ml.

DNA Ligase IV Antibody - NBP1-87405 from Novus Biologicals

Prices	$419.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

DNA Ligase IV Antibody - NBP2-16182 from Novus Biologicals

Prices	$329.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

Western Blot: DNA Ligase IV Antibody [NBP2-16182] - Mouse tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with DNA ligase IV antibody diluted at 1:500.

DNA Ligase IV Antibody - NB100-92452 from Novus Biologicals

Prices	$349.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

Western Blot: DNA Ligase IV Antibody [NB100-92452] - Western blot (WB) analysis of DNA Ligase IV (G619) antibody in extracts from Jurkat cells.

DNA Ligase IV Antibody (2D2) - H00003981-M02 from Novus Biologicals

Prices	$359.00
Sizes	100 µg
Host	Mouse
Clonality	Monoclonal
Clone	2D2

Sandwich ELISA: DNA Ligase IV Antibody (2D2) [H00003981-M02] - Detection limit for recombinant GST tagged LIG4 is approximately 1ng/ml as a capture antibody.

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