Anti-DNA Polymerase theta antibody
| Name | Anti-DNA Polymerase theta antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab80906 |
| Prices | $370.00 |
| Sizes | 50 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | WB ELISA ICC/IF ICC/IF |
| Species Reactivities | Human, Mouse, Rat, Rabbit, Horse, Guinea Pig, Bovine, Cat, Dog |
| Antigen | Synthetic peptide: SFTSSDDIAE VLFLELKLPP NREMKNQGSK KTLGSTRRGI DNGRKLRLGR , corresponding to C terminal amino acids 2125-2174 of Human DNA Polymerase theta Run BLAST with Run BLAST with |
| Description | Rabbit Polyclonal |
| Gene | POLQ |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Anti-DNA Polymerase theta antibody (ab80906) at 1 µg/ml + placenta lysate at 10 µgSecondaryHRP conjugated anti-Rabbit IgG at 1/50000 dilution
ICC/IF image of ab80906 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab80906, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.