Anti-DNA2 antibody
| Name | Anti-DNA2 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab96488 |
| Prices | $382.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | WB IHC-P |
| Species Reactivities | Human, Mouse, Rat |
| Antigen | Synthetic peptide conjugated to KLH derived from within residues 1000 to the C-terminus of Human DNA2 |
| Description | Rabbit Polyclonal |
| Gene | DNA2 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
All lanes : Anti-DNA2 antibody (ab96488) at 1 µg/mlLane 1 : HEK293 (Human embryonic kidney cell line) Whole Cell LysateLane 2 : HEK293 (Human embryonic kidney cell line) Nuclear Lysate Lane 3 : U2OS (Human osteosarcoma cell line) Whole Cell LysateLysates/proteins at 10 µg per lane.SecondaryGoat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.
All lanes : Anti-DNA2 antibody (ab96488)Lane 1 : U2OS cells plus control shRNALane 2 : U2OS cells plus shDNA2-1 shRNALane 3 : U2OS cells plus shDNA2-2 shRNASecondaryAnti-rabbit at 1/3000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.
IHC image of ab96488 staining in human liver carcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab96488, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Product References
Genomic analysis of primordial dwarfism reveals novel disease genes. - Genomic analysis of primordial dwarfism reveals novel disease genes.
Shaheen R, Faqeih E, Ansari S, Abdel-Salam G, Al-Hassnan ZN, Al-Shidi T, Alomar R, Sogaty S, Alkuraya FS. Genome Res. 2014 Feb;24(2):291-9.
DNA2 cooperates with the WRN and BLM RecQ helicases to mediate long-range DNA end - DNA2 cooperates with the WRN and BLM RecQ helicases to mediate long-range DNA end
Sturzenegger A, Burdova K, Kanagaraj R, Levikova M, Pinto C, Cejka P, Janscak P. J Biol Chem. 2014 Sep 26;289(39):27314-26.
Okazaki fragment processing-independent role for human Dna2 enzyme during DNA - Okazaki fragment processing-independent role for human Dna2 enzyme during DNA
Duxin JP, Moore HR, Sidorova J, Karanja K, Honaker Y, Dao B, Piwnica-Worms H, Campbell JL, Monnat RJ Jr, Stewart SA. J Biol Chem. 2012 Jun 22;287(26):21980-91.
CtIP-dependent DNA resection is required for DNA damage checkpoint maintenance - CtIP-dependent DNA resection is required for DNA damage checkpoint maintenance
Kousholt AN, Fugger K, Hoffmann S, Larsen BD, Menzel T, Sartori AA, Sorensen CS. J Cell Biol. 2012 Jun 25;197(7):869-76.
The mammalian proteins MMS19, MIP18, and ANT2 are involved in cytoplasmic - The mammalian proteins MMS19, MIP18, and ANT2 are involved in cytoplasmic
van Wietmarschen N, Moradian A, Morin GB, Lansdorp PM, Uringa EJ. J Biol Chem. 2012 Dec 21;287(52):43351-8.
Involvement of DNA ligase III and ribonuclease H1 in mitochondrial DNA - Involvement of DNA ligase III and ribonuclease H1 in mitochondrial DNA
Ruhanen H, Ushakov K, Yasukawa T. Biochim Biophys Acta. 2011 Dec;1813(12):2000-7. doi: