Anti-DNAJB6 antibody [EPR17122] - N-terminal

• Supplier: Abcam
• Catalog: ab198995
• Prices: $347.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: EPR17122
• Applications: WB FC ICC/IF ICC/IF IHC-P
• Species Reactivities: Mouse, Rat, Human
• Antigen: Recombinant fragment within Human DNAJB6 aa 1-100 (N terminal)
• Description: Rabbit Monoclonal
• Gene: DNAJB6
• Conjugate: Unconjugated

Product images

All lanes : Anti-DNAJB6 antibody [EPR17122] - N-terminal (ab198995) at 1/1000 dilutionLane 1 : U87-MG cell lysateLane 2 : SH-SY5Y cell lysateLane 3 : HeLa cell lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Anti-DNAJB6 antibody [EPR17122] - N-terminal (ab198995) at 1/10000 dilution + Jurkat cell lysate at 20 µgSecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

All lanes : Anti-DNAJB6 antibody [EPR17122] - N-terminal (ab198995) at 1/1000 dilutionLane 1 : Mouse heart lysateLane 2 : Rat brain lysateLane 3 : Rat heart lysateLane 4 : C6 cell lysateLane 5 : RAW264.7 cell lysateLane 6 : PC-12 cell lysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Immunohistochemical analysis of paraffin-embedded human testis tissue sections labeling DNAJB6 with ab198995 at a 1/250 dilution. Goat anti-rabbit IgG H&L (HRP) ab97051 used as the secondary at a 1/500 dilution. Counterstain hematoxylin.

Immunohistochemical analysis of paraffin-embedded rat cerebral cortex tissue sections labeling DNAJB6 with ab198995 at a 1/250 dilution. Goat anti-rabbit IgG H&L (HRP) ab97051 used as the secondary at a 1/500 dilution. Counterstain hematoxylin.

Immunocytochemistry/ Immunofluorescence analysis of SH-SY5Y cell line labeling DNAJB6 with ab198995 at a 1/250 dilution. Cells were fixed with 4% paraformaldehyde and permeabilized using 0.1% Triton X-100. ab150077 AlexaFluor®488 Goat anti-Rabbit was used as the secondary at a 1/500 dilution. Counterstained using ab7291 anti-Tubulin (mouse mAb) at a 1/1000 dilution with ab150120 AlexaFluor®594 Goat anti-Mouse secondary at a 1/500 dilution.

Flow cytometry analysis of DNAJB6 expression in 2% paraformaldehyde-fixed Jurkat (Human acute T cell leukemia) cells cells using ab198995 at 1/500 dilution (red), a Rabbit monoclonal IgG (ab172730) IgG isotype control (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). A FITC-conjugated goat anti-rabbit IgG (1/150) was used as the secondary antibody.

DNAJB6 was immunoprecipitated from Jurkat (Human acute T cell leukemia) whole cell extract with ab198995 at 1/150 dilution. Western blot was performed from the immunoprecipitate using ab198995 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.Lane 1: Jurkat (Human acute T cell leukemia) whole cell extract (Input) 10 µg. Lane 2: ab198995 IP in Jurkat (Human acute T cell leukemia) whole cell extract. Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab198995 in Jurkat (Human acute T cell leukemia) whole cell extract.Blocking and dilution buffer and concentration: 5% NFDM/TBST.