Anti-DNase II antibody
| Name | Anti-DNase II antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab8119 |
| Prices | $370.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | ICC/IF ICC/IF IHC-P WB |
| Species Reactivities | Human |
| Antigen | Peptide corresponding to amino acids 347 to 360 of human DNase II precursor |
| Description | Rabbit Polyclonal |
| Gene | DNASE2 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
All lanes : Anti-DNase II antibody (ab8119) at 1/500 dilutionLane 1 : Human spleen tissue lysate with absence of blocking peptideLane 2 : Human spleen tissue lysate with presence of blocking peptide
ab8119 at 5µg/ml staining DNase II in human spleen tissue by IHC
ICC/IF image of ab8119 stained Hek293 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab8119, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.