Anti-Dnmt1 antibody [60B1220.1] - ChIP Grade
| Name | Anti-Dnmt1 antibody [60B1220.1] - ChIP Grade |
|---|---|
| Supplier | Abcam |
| Catalog | ab92453 |
| Prices | $392.00 |
| Sizes | 100 µg |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Clone | 60B1220.1 |
| Applications | FC WB IHC-P ChIP IP |
| Species Reactivities | Mouse, Human, Zebrafish, Sheep, Goat, Bovine, Pig |
| Antigen | Synthetic peptide: EKDDREDKENAFKR , corresponding to amino acids 637-650 of Human Dnmt1 (NP_001370) |
| Description | Mouse Monoclonal |
| Gene | DNMT1 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
![All lanes : Anti-Dnmt1 antibody [60B1220.1] - ChIP Grade (ab92453)Lane 1 : 2102EP (human embryonic carcinoma) with absence of blocking peptideLane 2 : 2102EP (human embryonic carcinoma) with blocking peptideLane 3 : Recombinant human Dnmt1 protein with absence of blocking peptideLane 4 : Recombinant human Dnmt1 protein with blocking peptideLane 5 : NIH 3T3 (mouse embryonic fibroblast) with absence of blocking peptideLane 6 : NIH 3T3 (mouse embryonic fibroblast) with blocking peptideLane 7 : D3 (mouse embryonic stem cell) with absence of blocking peptideLane 8 : D3 (mouse embryonic stem cell) with blocking peptideSecondaryGoat anti-Mouse IgG HRP-conjugated secondary antibody](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_2/9458_Dnmt1-Primary-antibodies-ab92453-4.jpg)
All lanes : Anti-Dnmt1 antibody [60B1220.1] - ChIP Grade (ab92453)Lane 1 : 2102EP (human embryonic carcinoma) with absence of blocking peptideLane 2 : 2102EP (human embryonic carcinoma) with blocking peptideLane 3 : Recombinant human Dnmt1 protein with absence of blocking peptideLane 4 : Recombinant human Dnmt1 protein with blocking peptideLane 5 : NIH 3T3 (mouse embryonic fibroblast) with absence of blocking peptideLane 6 : NIH 3T3 (mouse embryonic fibroblast) with blocking peptideLane 7 : D3 (mouse embryonic stem cell) with absence of blocking peptideLane 8 : D3 (mouse embryonic stem cell) with blocking peptideSecondaryGoat anti-Mouse IgG HRP-conjugated secondary antibody
![Lane 1 : Anti-Dnmt1 antibody [60B1220.1] - ChIP Grade (ab92453) at 2 µg/mlLane 2 : Anti-Dnmt1 antibody [60B1220.1] - ChIP Grade (ab92453) at 0.2 µg/mlLane 1 : Mouse ES cell lysateLane 2 : Mouse NIH3T3 cell lysateSecondaryGoat anti-Mouse Ig HRP conjugated](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_2/9459_Dnmt1-Primary-antibodies-ab92453-3.jpg)
Lane 1 : Anti-Dnmt1 antibody [60B1220.1] - ChIP Grade (ab92453) at 2 µg/mlLane 2 : Anti-Dnmt1 antibody [60B1220.1] - ChIP Grade (ab92453) at 0.2 µg/mlLane 1 : Mouse ES cell lysateLane 2 : Mouse NIH3T3 cell lysateSecondaryGoat anti-Mouse Ig HRP conjugated
ab92453, at 2 µg/ml, staining Dnmt1 on formalin fixed, paraffin embedded medullar kidney tissue sections by Immunohistochemistry.
![Overlay histogram showing HeLa cells stained with ab92453 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab92453, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_2/9461_Dnmt1-Primary-antibodies-ab92453-13.jpg)
Overlay histogram showing HeLa cells stained with ab92453 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab92453, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
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