Anti-Dnmt3a antibody

• Supplier: Abcam
• Catalog: ab23565
• Prices: $398.00
• Sizes: 100 µg
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Applications: ICC/IF ICC/IF IHC-P WB
• Species Reactivities: Mouse, Rat, Human
• Antigen: Synthetic peptide conjugated to KLH derived from within residues 50 - 150 of Mouse Dnmt3a
• Blocking Peptide: Human Dnmt3a peptide
• Description: Rabbit Polyclonal
• Gene: DNMT3A
• Conjugate: Unconjugated

Product images

ab23565 staining Dnmt3a in mouse lung tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and blocked with 5% serum for 1 hour at 25°C; antigen retrieval was by heat mediation. Samples were incubated with primary antibody (1/300) for 24 hours at 4°C. A HRP-conjugated anti-rabbit IgG polyclonal (1/1000) was used as the secondary antibody.See Abreview

Anti-Dnmt3a antibody (ab23565) at 1 µg/ml + dnmt3a recombinant proteinSecondaryGoat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab7090)

All lanes : Anti-Dnmt3a antibody (ab23565) at 1/500 dilutionLane 1 : 40ug mouse brain tissue (transgenic mouse negative for Dnmt3a) - whole lysateLane 2 : 40ug mouse spleen tissue (higher than normal levels of Dnmt3a) - whole lysateSecondaryGoat anti-rabbit HRPdeveloped using the ECL techniquePerformed under reducing conditions.

All lanes : Anti-Dnmt3a antibody (ab23565) at 1 µg/mlLane 1 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell LysateLane 2 : HEK293 (Human embryonic kidney cell line) Whole Cell LysateLysates/proteins at 10 µg per lane.SecondaryGoat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.

ICC/IF image of ab23565 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab23565, 10µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Product References

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