Anti-Dnmt3b antibody

• Supplier: Abcam
• Catalog: ab16049
• Prices: $391.00
• Sizes: 100 µg
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Applications: IHC-P WB
• Species Reactivities: Mouse, Hamster, Rat, Human
• Antigen: Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Mouse Dnmt3b
• Blocking Peptide: Mouse Dnmt3b peptide
• Description: Rabbit Polyclonal
• Gene: DNMT3B
• Conjugate: Unconjugated

Product images

All lanes : Anti-Dnmt3b antibody (ab16049) at 1 µg/ml (blocked with 3% milk.)Lane 1 : HUES7 (Human embryonic stem cell line) Whole Cell Lysate Lane 2 : F9 (Mouse embryonic carcinoma cell line) Whole Cell Lysate Lane 3 : Mouse Embryonic Germ Cell Lysate Lane 4 : E14Tg2a (Mouse embryonic stem cell line) Whole Cell LysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilutionPerformed under reducing conditions.

All lanes : Anti-Dnmt3b antibody (ab16049) at 1 µg/mlLane 1 : CHO cell lysateLane 2 : CHO cell lysate with Mouse Dnmt3b peptide (ab16376) at 1 µg/mlLysates/proteins at 20 µg per lane.SecondaryGoat anti-rabbit Alexa fluor 680 at 1/5000 dilutionPerformed under reducing conditions.

All lanes : Anti-Dnmt3b antibody (ab16049) at 1/1000 dilutionLane 1 : Lysate prepared from human NCCIT cell chromatin fractionLane 2 : Lysate prepared from chromatin fraction of human NCCIT whole cellsLysates/proteins at 200000 cells per lane.SecondaryHRP-conjugated goat polyclonal to rabbit IgG (H+L) at 1/10000 dilutionPerformed under reducing conditions.

Product References

Effects of specific DNMT gene depletion on cancer cell transformation and breast - Effects of specific DNMT gene depletion on cancer cell transformation and breast

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DNA methyltransferase 3a expression decreases during apoptosis in primary - DNA methyltransferase 3a expression decreases during apoptosis in primary

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Ornithine decarboxylase antizyme induces hypomethylation of genome DNA and - Ornithine decarboxylase antizyme induces hypomethylation of genome DNA and

Yamamoto D, Shima K, Matsuo K, Nishioka T, Chen CY, Hu GF, Sasaki A, Tsuji T. PLoS One. 2010 Sep 3;5(9):e12554.

Loss of pericentromeric DNA methylation pattern in human glioblastoma is - Loss of pericentromeric DNA methylation pattern in human glioblastoma is

Fanelli M, Caprodossi S, Ricci-Vitiani L, Porcellini A, Tomassoni-Ardori F, Amatori S, Andreoni F, Magnani M, De Maria R, Santoni A, Minucci S, Pelicci PG. Oncogene. 2008 Jan 10;27(3):358-65. Epub 2007 Jul 23.

Fat10 is an epigenetic marker for liver preneoplasia in a drug-primed mouse model - Fat10 is an epigenetic marker for liver preneoplasia in a drug-primed mouse model

Oliva J, Bardag-Gorce F, French BA, Li J, McPhaul L, Amidi F, Dedes J, Habibi A, Nguyen S, French SW. Exp Mol Pathol. 2008 Apr;84(2):102-12.

RIP140 directs histone and DNA methylation to silence Ucp1 expression in white - RIP140 directs histone and DNA methylation to silence Ucp1 expression in white

Kiskinis E, Hallberg M, Christian M, Olofsson M, Dilworth SM, White R, Parker MG. EMBO J. 2007 Nov 28;26(23):4831-40. Epub 2007 Nov 1.