DNMT3B

Gene Symbol DNMT3B
Entrez Gene 1789
Alt Symbol ICF, ICF1, M.HsaIIIB
Species Human
Gene Type protein-coding
Description DNA (cytosine-5-)-methyltransferase 3 beta
Other Description DNA (cytosine-5)-methyltransferase 3B|DNA MTase HsaIIIB|DNA methyltransferase HsaIIIB
Swissprots Q9UKA6 Q9UJQ5 A2A2E2 B4DSM8 E1P5M7 Q9UBD4 Q9Y5S0 Q9UBC3 B4DSU1 Q9Y5R9 E7EN63 E9PBF2 Q9UNE5 E1P5M6
Accessions EAW76350 EAW76351 EAW76352 EAW76353 EAW76354 EAW76355 EAW76356 Q9UBC3 AB208880 BAD92117 AF129267 AAD31432 AF129268 AAD31433 AF129269 AAD31434 AF156487 AAD53062 AF156488 AAD53063 AF176228 AAF04015 AF331857 AAL57040 AK001191 BAG50865 AK295176 BAG58185 AK299776 BAG61659 AK299821 BAG61690 AK299915 BAG61753 AK300857 BAG62507 BC111933 AAI11934 BC118502 AAI18503 DQ321787 ABC48951 L32168 XM_011528653 XP_011526955 XM_011528654 XP_011526956 XR_936510 XR_936511 XR_936512 NM_001207055 NP_001193984 NM_001207056 NP_001193985 NM_006892 NP_008823 NM_175848 NP_787044 NM_175849 NP_787045 NM_175850 NP_787046
Function Required for genome-wide de novo methylation and is essential for the establishment of DNA methylation patterns during development. DNA methylation is coordinated with methylation of histones. May preferentially methylates nucleosomal DNA within the nucleosome core region. May function as transcriptional co- repressor by associating with CBX4 and independently of DNA methylation. Seems to be involved in gene silencing (By similarity). In association with DNMT1 and via the recruitment of CTCFL/BORIS, involved in activation of BAG1 gene expression by modulating dimethylation of promoter histone H3 at H3K4 and H3K9. Isoforms 4 and 5 are probably not functional due to the deletion of two conserved methyltransferase motifs. Function as transcriptional corepressor by associating with ZHX1. {ECO:0000250, ECO:0000269|PubMed:16357870, ECO:0000269|PubMed:17303076, ECO:0000269|PubMed:18413740, ECO:0000269|PubMed:18567530}.
Subcellular Location Nucleus {ECO:0000269|PubMed:11735126, ECO:0000269|PubMed:12145218}.
Tissue Specificity Ubiquitous; highly expressed in fetal liver, heart, kidney, placenta, and at lower levels in spleen, colon, brain, liver, small intestine, lung, peripheral blood mononuclear cells, and skeletal muscle. Isoform 1 is expressed in all tissues except brain, skeletal muscle and PBMC, 3 is ubiquitous, 4 is expressed in all tissues except brain, skeletal muscle, lung and prostate and 5 is detectable only in testis and at very low level in brain and prostate.
Top Pathways Cysteine and methionine metabolism, MicroRNAs in cancer

DNMT3B Antibody - 2161 from Cell Signaling Technology

Host Rabbit
Clonality Polyclonal

Dnmt3b (G-9) - sc-376043 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Dnmt3b (G-9): sc-376043. Western blot analysis of Dnmt3b expression in K-562 nuclear extract.

Dnmt3b (F-2) - sc-393845 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Dnmt3b (F-2): sc-393845. Western blot analysis of Dnmt3b expression in non-transfected: sc-110760 (A) and human Dnmt3b transfected: sc-128485 (B) 293 whole cell lysates and K-562 (C) and Jurkat (D) nuclear extracts.

Dnmt3b (N-19) - sc-10235 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
Dnmt3b (N-19): sc-10235. Western blot analysis of Dnmt3b expression in K-562 nuclear extract.

Dnmt3b (D-1) - sc-393279 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Dnmt3b (D-1): sc-393279. Western blot analysis of Dnmt3b expression in non-transfected: sc-110760 (A) and human Dnmt3b transfected: sc-128485 (B) 293 whole cell lysates.

Dnmt3b (T-16) - sc-10236 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
Dnmt3b (T-16): sc-10236. Western blot analysis of Dnmt3b expression in K-562 nuclear extract.

Dnmt3b (H-230) - sc-20704 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal

Dnmt3b (Q-25) - sc-130740 from Santa Cruz Biotechnology

Prices $279.00
Sizes 100 µg/ml
Host Rabbit
Clonality Polyclonal
Dnmt3b (Q-25): sc-130740. Immunoperoxidase staining of formalin-fixed, paraffin-embedded human prostate carcinoma tissue showing nuclear and cytoplasmic localization.

Dnmt3b (52A1018) - sc-52922 from Santa Cruz Biotechnology

Prices $279.00
Sizes 100 µg/ml
Host Mouse
Clonality Monoclonal
Dnmt3b (52A1018): sc-52922. Western blot analysis of Dnmt3b expression in non-transfected: sc-117752 (A) and human Dnmt3b transfected: sc-128484 (B) 293T whole cell lysates and K-562 nuclear extract (C).

Dnmt3b (2280C3a) - sc-81252 from Santa Cruz Biotechnology

Prices $295.00
Sizes 100 µg/ml
Host Mouse
Clonality Monoclonal
Dnmt3b (2280C3a): sc-81252 Western Blot analysis of Dnmt3b expression in F2408 whole cell lysate.

Dnmt3b (4H84) - sc-70984 from Santa Cruz Biotechnology

Prices $279.00
Sizes 100 µg/ml
Host Mouse
Clonality Monoclonal
Dnmt3b (4H84): sc-70984. Western blot analysis of Dnmt3b expression in non-transfected: sc-117752 (A) and human Dnmt3b transfected: sc-128484 (B) 293T whole cell lysates.

Anti-Dnmt3b antibody - ab16049 from Abcam

Prices $391.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-Dnmt3b antibody (ab16049) at 1 µg/ml (blocked with 3% milk.)Lane 1 : HUES7 (Human embryonic stem cell line) Whole Cell Lysate Lane 2 : F9 (Mouse embryonic carcinoma cell line) Whole Cell Lysate Lane 3 : Mouse Embryonic Germ Cell Lysate Lane 4 : E14Tg2a (Mouse embryonic stem cell line) Whole Cell LysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilutionPerformed under reducing conditions.

Anti-Dnmt3b antibody - ab71747 from Abcam

Host Rabbit
Clonality Polyclonal
Anti-Dnmt3b antibody (ab71747) at 1/100 dilution + T47-D cell lysate at 12.5 µgSecondaryHRP anti rabbit

Anti-Dnmt3b antibody - ab119282 from Abcam

Prices $384.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
ab119282, at 10 µg/ml, staining Dnmt3b in Human testis tissue by immunohistochemistry.

Anti-Dnmt3b antibody [52A1018] - ChIP Grade - ab176166 from Abcam

Prices $379.00
Sizes 50 µg
Host Mouse
Clonality Monoclonal
Clone 52A1018
All lanes : Anti-Dnmt3b antibody [52A1018] - ChIP Grade (ab176166) at 4 µg/mlLane 1 : NIH-3T3 cell lysateLane 2 : Recombinant Human Dnmt3b protein

Anti-Dnmt3b antibody [52A1018] - ChIP Grade - ab13604 from Abcam

Prices $401.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 52A1018
All lanes : Anti-Dnmt3b antibody [52A1018] - ChIP Grade (ab13604) at 2 µg/mlLane 1 : Cell lysates prepared from mouse Dnmt3a transfected 293 cells.Lane 2 : Cell lysates prepared from mouse Dnmt3b transfected 293 cellsLysates/proteins at 10 µg per lane.Observed band size : 110 kDa (why is the actual band size different from the predicted?)

Anti-Dnmt3b antibody [EPR3523] - ab79822 from Abcam

Prices $385.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR3523
All lanes : Anti-Dnmt3b antibody [EPR3523] (ab79822) at 1/1000 dilutionLane 1 : Flag-tagged Dnmt3bLane 2 : Dnmt3b knockout mouse embryonic stem cell extractLane 3 : Wild type mouse embryonic stem cell extractLane 4 : Wild type mouse embryonic stem cell extractLysates/proteins at 40 µg per lane.SecondaryDonkey anti-rabbit IgG conjugated to HRP (NA934) at 1/1000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.

Anti-Dnmt3b antibody - ChIP Grade - ab2851 from Abcam

Prices $394.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
ab2851 labellling Dnmt3b in the nucleus of Mouse brain tissue (right) compared with a negative control (left) by Immunohistochemistry (formalin/PFA-fixed paraffin-embedded sections). To expose target proteins, antigen retrieval method was performed using 10mM sodium citrate (pH 6.0) microwaved for 8-15 min. Tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS. Tissue sections were incubated with primary antibody (1:200 in 3% BSA-PBS) overnight at 4°C. A HRP-conjugated anti-rabbit was used as the secondary antibody, followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.

Anti-DNMT3B Antibody (aa647-696) - LS-C111224 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
DNMT3B antibody LS-C111224 Western blot of OVCAR-3 cell lysate.

Anti-DNMT3B Antibody (aa395-425) - LS-C320919 from LifeSpan Bioscience

Prices $345.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-DNMT3B Antibody - LS-C341188 from LifeSpan Bioscience

Prices $385.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-DNMT3B Antibody (C-Terminus) - LS-C305955 from LifeSpan Bioscience

Prices $460.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Western blot of DNMT3B antibody LS-X234967.

Anti-DNMT3B Antibody (Internal, clone EPR3523) - LS-C105552 from LifeSpan Bioscience

Prices $485.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR3523
Western blot analysis on (A) A549, (B) HeLa and (C) A431 cell lysates using anti-Dnmt3b antibody (LS-C105552), dilution: 1:5000.

Anti-DNMT3B Antibody - LS-C332279 from LifeSpan Bioscience

Prices $255.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts of various tissues, using DNMT3B antibody.

Anti-DNMT3B Antibody (aa389-417) - LS-C96548 from LifeSpan Bioscience

Prices $295.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
Formalin-fixed and paraffin-embedded human prostate carcinoma tissue reacted with Dnmt3b antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
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