Anti-Dnmt3b antibody [52A1018] - ChIP Grade
| Name | Anti-Dnmt3b antibody [52A1018] - ChIP Grade |
|---|---|
| Supplier | Abcam |
| Catalog | ab13604 |
| Prices | $401.00 |
| Sizes | 100 µg |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Clone | 52A1018 |
| Applications | ChIP IHC-P WB IP ICC/IF ICC/IF FC |
| Species Reactivities | Mouse, Rat, Human |
| Antigen | His-tagged recombinant protein (Mouse) |
| Description | Mouse Monoclonal |
| Gene | DNMT3B |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
![All lanes : Anti-Dnmt3b antibody [52A1018] - ChIP Grade (ab13604) at 2 µg/mlLane 1 : Cell lysates prepared from mouse Dnmt3a transfected 293 cells.Lane 2 : Cell lysates prepared from mouse Dnmt3b transfected 293 cellsLysates/proteins at 10 µg per lane.Observed band size : 110 kDa (why is the actual band size different from the predicted?)](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_2/9522_ab13604.jpg)
All lanes : Anti-Dnmt3b antibody [52A1018] - ChIP Grade (ab13604) at 2 µg/mlLane 1 : Cell lysates prepared from mouse Dnmt3a transfected 293 cells.Lane 2 : Cell lysates prepared from mouse Dnmt3b transfected 293 cellsLysates/proteins at 10 µg per lane.Observed band size : 110 kDa (why is the actual band size different from the predicted?)
Ab13604 staining Human normal testis. Staining is localised to the nucleus.Left panel: with primary antibody at 4 ug/ml. Right panel: isotype control.Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the DAKO 3-in-1 antigen retrieval buffer EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
ICC/IF image of ab13604 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab13604, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
![Overlay histogram showing HepG2 cells stained with ab13604 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab13604, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells ) used under the same conditions. Acquisition of >5,000 events was performed.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_2/9525_Dnmt3b-Primary-antibodies-ab13604-3.jpg)
Overlay histogram showing HepG2 cells stained with ab13604 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab13604, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells ) used under the same conditions. Acquisition of >5,000 events was performed.
Product References
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