Anti-Dopamine Receptor D3 antibody
| Name | Anti-Dopamine Receptor D3 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab42114 |
| Prices | $415.00 |
| Sizes | 50 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | ICC/IF ICC/IF WB ELISA DB IP |
| Species Reactivities | Mouse, Rat, Human |
| Antigen | Synthetic peptide: CQACHVSPELYRATTWGY Run BLAST with Run BLAST with |
| Blocking Peptide | Dopamine Receptor D3 peptide |
| Description | Rabbit Polyclonal |
| Gene | DRD3 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Anti-Dopamine Receptor D3 antibody (ab42114) at 1/750 dilution + PC-D3R sample
ICC/IF image of ab42114 stained PC12 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab42114, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Product References
Dopamine synthesis and D3 receptor activation in pancreatic beta-cells regulates - Dopamine synthesis and D3 receptor activation in pancreatic beta-cells regulates
Ustione A, Piston DW. Mol Endocrinol. 2012 Nov;26(11):1928-40.
Dopamine receptor mechanisms mediate corticotropin-releasing factor-induced - Dopamine receptor mechanisms mediate corticotropin-releasing factor-induced
Krishnan B, Centeno M, Pollandt S, Fu Y, Genzer K, Liu J, Gallagher JP, Shinnick-Gallagher P. Eur J Neurosci. 2010 Mar;31(6):1027-42.