Anti-Dopamine Receptor D4 antibody
| Name | Anti-Dopamine Receptor D4 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab20424 |
| Prices | $370.00 |
| Sizes | 50 µl |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | ICC/IF WB IHC-P |
| Species Reactivities | Rat |
| Antigen | D4 (Ac-176-185) covalently attached to a carrier protein |
| Blocking Peptide | Dopamine Receptor D4 peptide |
| Description | Rabbit Polyclonal |
| Gene | Drd4 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat gastric tissue labelling Dopamine Receptor D4 with ab20424 at 1/1000. Dopamine Receptor D4 was not found in the gastric mucosa (e - left) but was observed in the gastric enteric plexus (f - right). Paraffin sections were baked at 60°C for 2 hours, then deparaffinized in xylene for 20 minutes. Slides were rehydrated in descending concentrations of alcohol and transferred from 70% alcohol solution to distilled water for rinsing. Before staining, slides were pretreated with 10 mM citric acid (pH 6.0) at 92–98°C for 10 min and incubated in 10% normal serum and 0.1% Triton X-100 for 30 min. The sections were then incubated with the primary antibody, followed by the appropriate secondary antibody. Sections were washed in PBS and mounted in 90% glycerin PBS.
ab20424 stained PC12 cells. The cells were 4% formaldhyde fixed for 10 minutes at room temperature and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1hour at room temperature to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab20424 at 1/100) overnight at +4°C. The secondary antibody (pseudo-colored green) was Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (ab150081) used at a 1/1000 dilution for 1hour at room temperature. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1hour at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.
Product References
Cellular localization of dopamine receptors in the gastric mucosa of rats. - Cellular localization of dopamine receptors in the gastric mucosa of rats.
Wang Q, Ji T, Zheng LF, Feng XY, Wang ZY, Lian H, Song J, Li XF, Zhang Y, Zhu JX. Biochem Biophys Res Commun. 2012 Jan 6;417(1):197-203. doi:
Dopamine receptor mechanisms mediate corticotropin-releasing factor-induced - Dopamine receptor mechanisms mediate corticotropin-releasing factor-induced
Krishnan B, Centeno M, Pollandt S, Fu Y, Genzer K, Liu J, Gallagher JP, Shinnick-Gallagher P. Eur J Neurosci. 2010 Mar;31(6):1027-42.