All lanes : Anti-DPP1 antibody [EPR7749] - C-terminal (ab192019) at 1/1000 dilutionLane 1 : HT29 cell lysatesLane 2 : JAR cell lysatesLane 3 : A549 cell lysatesLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilutiondeveloped using the ECL technique
Anti-DPP1 antibody [EPR7749] - C-terminal (ab192019) at 1/1000 dilution + Human DPP1 recombinant protein at 0.01 µgSecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilutiondeveloped using the ECL technique
Flow cytometric analysis of 2% paraformaldehyde fixed A549 cells labeling DPP1 with ab192019 at a 1/340 dilution (red) compared to a Rabbit monoclonal IgG isotype control (green). Secondary staining was with Goat anti Rabbit IgG (FITC) at 1/50 dilution.