ab126352 staining DPY30 in Mouse NIH/3T3 cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.2% Tween 20 in PBS and blocked with 3% BSA for 30 minutes at 25°C. Samples were incubated with primary antibody (2μg/ml in 3% BSA + 0.2% Tween 20 + PBS) for 16 hours at 4°C. An Alexa Fluor® 488-conjugated Goat anti-rabbit IgG polyclonal (4μg/ml in blocking solution for 1 hour) was used as the secondary antibody.See Abreview
ab126352 at a 1/200 dilution staining DPY30 in paraffin embedded uman bronchus tissue by Imunohistochemistry.
ab126352 at 4 µg/ml staining DPY30 in in PFA/Triton X-100 fixed/permeabilized U251 cells by Immunofluorescence (green).