Anti-DPYD antibody
| Name | Anti-DPYD antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab54797 |
| Prices | $390.00 |
| Sizes | 100 µg |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Applications | WB ICC/IF ICC/IF IHC-P FC |
| Species Reactivities | Human |
| Antigen | Recombinant fragment, corresponding to amino acids 1-111 of Human DPYD |
| Description | Mouse Monoclonal |
| Gene | DPYD |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ab54797 at 10 ug/ml staining DPYD in human Hela cells by Immunocytochemistry / Immunofluorescence.
![Overlay histogram showing HepG2 cells stained with ab54797 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab54797, 1µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HepG2 cells fixed with 100% methanol (5 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_2/10087_DPYD-Primary-antibodies-ab54797-2.jpg)
Overlay histogram showing HepG2 cells stained with ab54797 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab54797, 1µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HepG2 cells fixed with 100% methanol (5 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.
Product References
microRNAs miR-27a and miR-27b directly regulate liver dihydropyrimidine - microRNAs miR-27a and miR-27b directly regulate liver dihydropyrimidine
Offer SM, Butterfield GL, Jerde CR, Fossum CC, Wegner NJ, Diasio RB. Mol Cancer Ther. 2014 Mar;13(3):742-51.
Comparative functional analysis of DPYD variants of potential clinical relevance - Comparative functional analysis of DPYD variants of potential clinical relevance
Offer SM, Fossum CC, Wegner NJ, Stuflesser AJ, Butterfield GL, Diasio RB. Cancer Res. 2014 May 1;74(9):2545-54.
Phenotypic profiling of DPYD variations relevant to 5-fluorouracil sensitivity - Phenotypic profiling of DPYD variations relevant to 5-fluorouracil sensitivity
Offer SM, Wegner NJ, Fossum C, Wang K, Diasio RB. Cancer Res. 2013 Mar 15;73(6):1958-68.
Astrocyte elevated gene-1 regulates hepatocellular carcinoma development and - Astrocyte elevated gene-1 regulates hepatocellular carcinoma development and
Yoo BK, Emdad L, Su ZZ, Villanueva A, Chiang DY, Mukhopadhyay ND, Mills AS, Waxman S, Fisher RA, Llovet JM, Fisher PB, Sarkar D. J Clin Invest. 2009 Mar;119(3):465-77.
Identification of genes conferring resistance to 5-fluorouracil. - Identification of genes conferring resistance to 5-fluorouracil.
Yoo BK, Gredler R, Vozhilla N, Su ZZ, Chen D, Forcier T, Shah K, Saxena U, Hansen U, Fisher PB, Sarkar D. Proc Natl Acad Sci U S A. 2009 Aug 4;106(31):12938-43. doi: