All lanes : Anti-DR1 antibody [EPR13122] (ab180164) at 1/1000 dilutionLane 1 : HeLa cell lysateLane 2 : 293T cell lysateLane 3 : Jurkat cell lysateLane 4 : Human testis lysateLysates/proteins at 10 µg per lane.developed using the ECL technique
Immunohistochemical analysis of paraffin-embedded Human ovarian carcinoma tissue labeling DR1 using ab180164 at 1/250.
Immunohistochemical analysis of paraffin-embedded Human prostate tissue labeling DR1 using ab180164 at 1/250.
Western blot analysis on immunoprecipitation pellet from (1) 293T cell lysate or (2) 1X PBS (negative control) pulling down DR1 using ab180164 at 1/10 and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.