Anti-DR4 antibody [32A242]
| Name | Anti-DR4 antibody [32A242] |
|---|---|
| Supplier | Abcam |
| Catalog | ab13890 |
| Prices | $382.00 |
| Sizes | 100 µg |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Clone | 32A242 |
| Applications | FC WB |
| Species Reactivities | Human |
| Antigen | Peptide corresponding to amino acid 1 to 20 of human DR4 mature protein |
| Description | Mouse Monoclonal |
| Gene | TNFRSF10A |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
![Overlay histogram showing Jurkat cells stained with ab13890 (red line). The cells were fixed with 80% methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab13890, 1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive result in 4% paraformaldehyde (10 min) fixed Jurkat cells used under the same conditions. Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_2/10141_ab13890-2-ab13890FC.jpg)
Overlay histogram showing Jurkat cells stained with ab13890 (red line). The cells were fixed with 80% methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab13890, 1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive result in 4% paraformaldehyde (10 min) fixed Jurkat cells used under the same conditions. Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.
Product References
Regulation in the targeting of TRAIL receptor 1 to cell surface via GODZ for - Regulation in the targeting of TRAIL receptor 1 to cell surface via GODZ for
Oh Y, Jeon YJ, Hong GS, Kim I, Woo HN, Jung YK. Cell Death Differ. 2012 Jul;19(7):1196-207.
Peroxiredoxin 6 interferes with TRAIL-induced death-inducing signaling complex - Peroxiredoxin 6 interferes with TRAIL-induced death-inducing signaling complex
Choi H, Chang JW, Jung YK. Cell Death Differ. 2011 Mar;18(3):405-14.
Scatter factor protects tumor cells against apoptosis caused by TRAIL. - Scatter factor protects tumor cells against apoptosis caused by TRAIL.
Fan S, Meng Q, Laterra JJ, Rosen EM. Anticancer Drugs. 2010 Jan;21(1):10-24.
Inhibition of constitutive activation of STAT3 by curcurbitacin-I (JSI-124) - Inhibition of constitutive activation of STAT3 by curcurbitacin-I (JSI-124)
Ishdorj G, Johnston JB, Gibson SB. Mol Cancer Ther. 2010 Dec;9(12):3302-14.
Death receptor-4 (DR4) expression is regulated by transcription factor NF-kappaB - Death receptor-4 (DR4) expression is regulated by transcription factor NF-kappaB
Mendoza FJ, Ishdorj G, Hu X, Gibson SB. Apoptosis. 2008 Jun;13(6):756-70.